Dataset Information

?-xylosidase and ?-mannosidase in combination improved growth performance and altered microbial profiles in weanling pigs fed a corn-soybean meal-based diet.

ABSTRACT: Objective:In this study, two glycosidases (XMosidases), ?-xylosidase and ?-mannosidase, were investigated on their in vitro hydrolysis activities of feed and on the improvement of growth performance in vivo in weanling pigs. Methods:Enzyme activities of XMosidases in vitro were evaluated in test tubes and simulation of gastric and small intestinal digestion, respectively, in the presence of NSPase. In vivo study was performed in 108 weaned piglets in a 28-d treatment. Pigs were allotted to one of three dietary treatments with six replicate pens in each treatment. The three treatment groups were as follows: i) Control (basal diet). ii) CE (basal diets + CE); iii) CE-Xmosidases (basal diets +CE + ?-xylosidase at 800U/kg and ?-mannosidase at 40U/kg). CE was complex enzymes (amylase, protease, xylanase and mannanase). Results:In vitro XMosidases displayed significant activities on hydrolysis of corn and soybean meal in the presence of non-starch polysaccharide degrading enzymes (xylanase and ?-mannanase). In vitro simulation of gastric and small intestinal digestion by XMosidases showed XMosidases achieved 67.89±0.22% of dry matter digestibility and 63.12±0.21% of energy digestibility at 40 °C for 5 hrs. In weanling pigs, additional XMosidases to CE in feed improved ADG, F:G (p< 0.05) and ATTD of crude protein (p= 0.01) and dry matter (p=0.02). XMosidases also altered the gut bacterial diversity and composition by increasing the proportion of beneficial bacteria. Conclusion:Addition of a complex enzyme supplementation (contained xylanase, ?-mannanase, protease and amylase), XMosidases (?-xylosidase and ?-mannosidase) can further improve the growth performance and nutrient digestion of young pigs.

SUBMITTER: Liu S

PROVIDER: S-EPMC6817776 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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β-xylosidase and β-mannosidase in combination improved growth performance and altered microbial profiles in weanling pigs fed a corn-soybean meal-based diet.

Liu Shaoshuai S Ma Chang C Liu Ling L Ning Dong D Liu Yajing Y Dong Bing B

Asian-Australasian journal of animal sciences 20190214 11

<h4>Objective</h4>In this study, two glycosidases (XMosidases), β-xylosidase and -mannosidase, were investigated on their in vitro hydrolysis activities of feed and on the improvement of growth performance in vivo in weanling pigs.<h4>Methods</h4>Enzyme activities of XMosidases in vitro were evaluated in test tubes and simulation of gastric and small intestinal digestion, respectively, in the presence of NSPase. In vivo study was performed in 108 weaned piglets in a 28-d treatment. Pigs were al ...[more]

PMID: 31010999

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Project description:Background:The objective of this study was to determine the effects of supplementing Cu on growth performance, Cu metabolism and Cu-related enzyme activities of weanling pigs fed diets with two different Cu sources, and to estimate optimal Cu requirements and relative bioavailability from these two sources for pigs. Methods:Weanling pigs were allocated to 14 treatments arranged factorially, including 6 added Cu levels (5, 10, 20, 40, 80, 160?mg/kg), and 2 mineral sources (tribasic Cu chloride, TBCC and copper proteinate, CuPro), as well as one negative control (0?mg/kg added Cu level) and one maximum allowed level treatment (200?mg/kg TBCC) for the entire 38-d experiment. Growth performance, mineral status and enzyme activities were measured at the end of this study. Results:Increasing levels of Cu showed linear and quadratic responses (P?< 0.01) for final BW, ADG and FCR regardless of the sources. Supplementation with TBCC (>?80?mg/kg) and CuPro (>?20?mg/kg) significantly decreased (P?< 0.05) diarrhea incidence of weanling pigs. There were linear and quadratic increases (P?< 0.01) in bile, hepatic, and intestinal Cu concentrations, fecal Cu contents, and plasma enzyme activities (alkaline phosphatase, ceruloplasmin, Cu, Zn-Superoxide dismutase (Cu/Zn SOD), and glutathione peroxidase), whereas plasma malondialdehyde decreased (P?< 0.01) linearly and quadratically as dietary Cu level increased. Similarly, pigs fed CuPro absorbed and retained more Cu and excreted less Cu than those fed TBCC when supplemented 80?mg/kg and above. Optimal dietary Cu requirements for pigs from 28 to 66 d of age estimated based on fitted broken-line models (P?< 0.05) of bile Cu, plasma Cu/Zn SOD and growth performance were 93-140?mg/kg from TBCC, and 63-98?mg/kg from CuPro accordingly. According to slope ratios from multiple linear regression, the bioavailability value of CuPro relative to TBCC (100%) was 156-263% (P?< 0.01). Conclusion:The findings indicated that Cu recommendation from current NRC (5-6?mg/kg) was not sufficient to meet the high requirement of weanling pigs. Cu from CuPro was significantly more bioavailable to weanling pigs than TBCC in stimulating growth and enzyme activities, decreasing diarrhea frequency and fecal Cu contents to the environment.

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Dataset Information

?-xylosidase and ?-mannosidase in combination improved growth performance and altered microbial profiles in weanling pigs fed a corn-soybean meal-based diet.

Publications

β-xylosidase and β-mannosidase in combination improved growth performance and altered microbial profiles in weanling pigs fed a corn-soybean meal-based diet.

Similar Datasets

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