Ontology highlight
ABSTRACT:
SUBMITTER: Hu X
PROVIDER: S-EPMC6820627 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Hu Xinlin X Yuan Xin X He Nisha N Zhuang Tony Z TZ Wu Pan P Zhang Guimin G
3 Biotech 20191029 11
Thermostable α-amylases are widely used in industry. The α-amylase from <i>Bacillus licheniformis</i> (BLA) with six potential glycosylation sites possessed excellent thermal and pH stability and high activity. Here, it was expressed in <i>Pichia pastoris</i>. The Pic-BLA-producing yeast without any antibiotics-resistant gene was cultivated in flasks and the amylase activity in fermentation supernatant reached 900 U/mL. The recombinant α-amylase Pic-BLA produced in <i>P. pastoris</i> was deeply ...[more]