Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores.

ABSTRACT: Nanopore sensing is a powerful single-molecule approach for the detection of biomolecules. Recent studies have demonstrated that aerolysin is a promising candidate to improve the accuracy of DNA sequencing and to develop novel single-molecule proteomic strategies. However, the structure-function relationship between the aerolysin nanopore and its molecular sensing properties remains insufficiently explored. Herein, a set of mutated pores were rationally designed and evaluated in silico by molecular simulations and in vitro by single-channel recording and molecular translocation experiments to study the pore structural variation, ion selectivity, ionic conductance and capabilities for sensing several biomolecules. Our results show that the ion selectivity and sensing ability of aerolysin ar

SUBMITTER: Cao C 

PROVIDER: S-EPMC6820719 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature