Ontology highlight
ABSTRACT:
SUBMITTER: Standage-Beier K
PROVIDER: S-EPMC6821290 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Standage-Beier Kylie K Tekel Stefan J SJ Brookhouser Nicholas N Schwarz Grace G Nguyen Toan T Wang Xiao X Brafman David A DA
Nucleic acids research 20191101 19
Current approaches to identify cell populations that have been modified with deaminase base editing technologies are inefficient and rely on downstream sequencing techniques. In this study, we utilized a blue fluorescent protein (BFP) that converts to green fluorescent protein (GFP) upon a C-to-T substitution as an assay to report directly on base editing activity within a cell. Using this assay, we optimize various base editing transfection parameters and delivery strategies. Moreover, we utili ...[more]