Direct Detection and Quantification of Neoantigens.
Ontology highlight
ABSTRACT:
SUBMITTER: Wang Q
PROVIDER: S-EPMC6825591 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Direct Detection and Quantification of Neoantigens.
Wang Qing Q Douglass Jacqueline J Hwang Michael S MS Hsiue Emily Han-Chung EH Mog Brian J BJ Zhang Ming M Papadopoulos Nickolas N Kinzler Kenneth W KW Zhou Shibin S Vogelstein Bert B
Cancer immunology research 20190916 11
Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute am ...[more]
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