Dataset Information

Effects of Mannan Oligosaccharides on Gas Emission, Protein and Energy Utilization, and Fasting Metabolism in Sheep.

ABSTRACT: This study investigated the effects of mannan oligosaccharides (MOS) on in vitro and in vivo gas emission, utilization of crude protein (CP) and energy, and relative parameters of sheep under fasting metabolism conditions. In vitro gas productions were evaluated over 12 h in sheep diets containing different amounts of MOS (from 0% to 6.0%/kg, the increment was 0.5%). A control experiment was used to assess the gas emission, utilization of CP and energy, and fasting metabolism in control sheep and sheep treated with 2.0% MOS over 24 days (d). The results showed that 2.0% MOS supplementation led to the lowest in vitro CO2 production and less CH4 production, while also leading to decrease in vivo nutrients intake, CP and energy excretion, digested and retained CP, and energy released as CH4 (p < 0.05). Furthermore, 2.0% MOS supplementation appeared to decrease in vivo O2 consumption and CH4 production per metabolic body weight (BW0.75), and increase the CP retention rate of sheep (p < 0.074). MOS did not affect other parameters, along with the same parameters of sheep under fasting metabolism conditions (p > 0.05). The findings indicate MOS has only slight effects on the gas emission and nutrients and energy metabolism of sheep.

SUBMITTER: Zheng C

PROVIDER: S-EPMC6826375 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Effects of Mannan Oligosaccharides on Gas Emission, Protein and Energy Utilization, and Fasting Metabolism in Sheep.

Zheng Chen C Ma Junjun J Liu Ting T Wei Bingdong B Yang Huaming H

Animals : an open access journal from MDPI 20190928 10

This study investigated the effects of mannan oligosaccharides (MOS) on in vitro and in vivo gas emission, utilization of crude protein (CP) and energy, and relative parameters of sheep under fasting metabolism conditions. In vitro gas productions were evaluated over 12 h in sheep diets containing different amounts of MOS (from 0% to 6.0%/kg, the increment was 0.5%). A control experiment was used to assess the gas emission, utilization of CP and energy, and fasting metabolis ...[more]

PMID: 31569418

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Project description:The objective of this study was to evaluate the effects of dietary supplementation with live yeast (Saccharomyces cerevisiae), mannan-oligosaccharides and the combination of these additives on the inflammatory response, ruminal parameters and rumen morphology of sheep fed a high grain-based diet. Thirty-Two Dorper x Santa Ines crossbred lambs with an average weight of 24±2 kg were distributed in a completely randomized design. The animals were housed in individual stalls and fed ad libitum. Diet treatments were: Control (without additive); LY (2 g/kg DM of live yeast, Saccharomyces cerevisiae), MOS (2 g/kg DM of mannan-oligosaccharides) and LY+MOS (2 g/kg DM of LY + 2 g/kg DM of MOS). The experiment lasted 42 days. The supplementation with MOS alone and the additives combination resulted in increased ruminal pH (P<0.01), while the total concentrations of short chain fatty acids (SCFA) in the rumen were higher (P<0.05) only in the diets with LY and MOS. Ammonia (NH3) concentration in the rumen decreased (P<0.04) with the additives usage. Diets with LY, MOS and with additives combination reduced (P<0.01) the levels of lipopolysaccharides (LPS) in the plasma with values of 0.46; 0.44 and 0.04 EU/mL, respectively when compared to the control (0.93 EU/mL). MOS and LY+MOS treatments had reduced stratum corneum thickness (P<0.01) in comparison to the control treatment. The total thickness of ruminal epithelium was lower with the addition of MOS in the diet (P<0.03) than with LY additive. The incidence and severity of hepatic abscesses in animals whose diet was supplemented with LY and LY+MOS was lower (P<0.05) than in animals fed the control diet. The use of LY, MOS and LY+MOS in the high-concentrate diets for sheep reduced NH3 concentrations and LPS translocation into the bloodstream. Diets containing MOS and LY+MOS enhanced the health of the ruminal epithelium by reducing the thickness of the stratum corneum, and diets containing LY and LY+MOS decreased the incidence and severity of hepatic abscesses.

Project description:Higher dietary energy is often used to achieve better animal performance in mutton sheep production. Notably, changing the diet formula affects rumen fermentation and the microbiota of ruminants. In this study, we investigated the effect of dietary energy on rumen fermentation and ruminal microbiota in fattening sheep. Fifteen 2-month-old white-headed Suffolk sheep (♂) × Hu sheep (♀) crossbred lambs were randomly divided into three treatments based on the dietary energy of the feeds fed: 8.67 MJ/kg (Low energy (LE); n = 5), 10.38 MJ/kg (standard energy (CON); n = 5), and 12.31 MJ/kg (high energy (HE); n = 5) groups. After 70 days of feeding, sheep were slaughtered and the ruminal fluids were collected and analyzed to determine fermentation parameters. Microbiota was determined using metagenomics sequencing. Notably, the microbial cell protein (MCP) and butyric acid concentrations were significantly high in the HE group. Metagenomic sequencing revealed that ACE and Chao indexes of the HE group were significantly decreased. Four genera among the major classified taxa across all the kingdoms differed in relative abundance in the three dietary energy levels. The relative abundances of Prevotella_brevis, Succiniclasticum_ruminis, Prevotellace-ae_bacterium, and Lachnospiraceae_bacterium were significantly correlated with rumen fermentation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis further revealed that a high-energy diet increased lipid metabolism of microbiota. The Carbohydrate Active enzymes (CAZy) gene, which participates in energy metabolism, was upregulated, while genes regulating plant cell wall degradation were downregulated in the HE group. These results suggest that a high-energy diet had minimal influence on the rumen fermentation pattern but altered the composition of the rumen microbiota, enhancing microbial lipid metabolism and limiting crude fiber metabolism. The findings of this study provide scientific evidence of the effect of dietary energy on ruminant fermentation and fattening sheep production.

Dataset Information

Effects of Mannan Oligosaccharides on Gas Emission, Protein and Energy Utilization, and Fasting Metabolism in Sheep.

Publications

Effects of Mannan Oligosaccharides on Gas Emission, Protein and Energy Utilization, and Fasting Metabolism in Sheep.

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