Dataset Information

Transferrin Identification in Sterlet (Acipenser ruthenus) Reproductive System.

ABSTRACT: Transferrins are a superfamily of iron-binding proteins and are recognized as multifunctional proteins. In the present study, transcriptomic and proteomic methods were used to identify transferrins in the reproductive organs and sperm of out-of-spawning and spermiating sterlet (Acipenser ruthenus) males. The results showed that seven transferrin transcripts were identified in the transcriptome of sterlet, and these transcripts were qualified as two different transferrin genes, serotransferrin and melanotransferrin, with several isoforms present for serotransferrin. The relative abundance of serotransferrin isoforms was higher in the kidneys and Wolffian ducts in the spermiating males compared to out-of-spawning males. In addition, transferrin was immunodetected in sterlet seminal plasma, but not in sterlet spermatozoa extract. Mass spectrometry identification of transferrin in seminal plasma but not in spermatozoa corroborates immunodetection. The identification of transferrin in the reproductive organs and seminal plasma of sterlet in this study provides the potential function of transferrin during sturgeon male reproduction.

SUBMITTER: Xin M

PROVIDER: S-EPMC6826671 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Transferrin Identification in Sterlet (<i>Acipenser ruthenus</i>) Reproductive System.

Xin Miaomiao M Vechtova Pavlina P Shaliutina-Kolesova Anna A Fussy Zoltan Z Loginov Dmitry D Dzyuba Borys B Linhart Otomar O Boryshpolets Serhii S Rodina Marek M Li Ping P Loginova Yana Y Sterba Jan J

Animals : an open access journal from MDPI 20190930 10

Transferrins are a superfamily of iron-binding proteins and are recognized as multifunctional proteins. In the present study, transcriptomic and proteomic methods were used to identify transferrins in the reproductive organs and sperm of out-of-spawning and spermiating sterlet (<i>Acipenser ruthenus</i>) males. The results showed that seven transferrin transcripts were identified in the transcriptome of sterlet, and these transcripts were qualified as two different transferrin genes, serotransfe ...[more]

PMID: 31575042

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Dataset Information

Transferrin Identification in Sterlet (Acipenser ruthenus) Reproductive System.

Publications

Transferrin Identification in Sterlet (<i>Acipenser ruthenus</i>) Reproductive System.

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