Dataset Information

C-terminal Redox Domain of Arabidopsis APR1 is a Non-Canonical Thioredoxin Domain with Glutaredoxin Function.

ABSTRACT: Sulfur is an essential nutrient that can be converted into utilizable metabolic forms to produce sulfur-containing metabolites in plant. Adenosine 5'-phosphosulfate (APS) reductase (APR) plays a vital role in catalyzing the reduction of activated sulfate to sulfite, which requires glutathione. Previous studies have shown that the C-terminal domain of APR acts as a glutathione-dependent reductase. The crystal structure of the C-terminal redox domain of Arabidopsis APR1 (AtAPR1) shows a conserved ?/? thioredoxin fold, but not a glutaredoxin fold. Further biochemical studies of the redox domain from AtAPR1 provided evidence to support the structural observation. Collectively, our results provide structural and biochemical information to explain how the thioredoxin fold exerts the glutaredoxin function in APR.

SUBMITTER: Chen FF

PROVIDER: S-EPMC6827007 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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C-terminal Redox Domain of <i>Arabidopsis</i> APR1 is a Non-Canonical Thioredoxin Domain with Glutaredoxin Function.

Chen Fang-Fang FF Chien Chia-Yu CY Cho Chao-Cheng CC Chang Yu-Yung YY Hsu Chun-Hua CH

Antioxidants (Basel, Switzerland) 20191008 10

Sulfur is an essential nutrient that can be converted into utilizable metabolic forms to produce sulfur-containing metabolites in plant. Adenosine 5'-phosphosulfate (APS) reductase (APR) plays a vital role in catalyzing the reduction of activated sulfate to sulfite, which requires glutathione. Previous studies have shown that the C-terminal domain of APR acts as a glutathione-dependent reductase. The crystal structure of the C-terminal redox domain of <i>Arabidopsis</i> APR1 (AtAPR1) shows a con ...[more]

PMID: 31597378

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Dataset Information

C-terminal Redox Domain of Arabidopsis APR1 is a Non-Canonical Thioredoxin Domain with Glutaredoxin Function.

Publications

C-terminal Redox Domain of <i>Arabidopsis</i> APR1 is a Non-Canonical Thioredoxin Domain with Glutaredoxin Function.

Similar Datasets

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