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Deficiency of calpain-6 inhibits primary ciliogenesis.

ABSTRACT: The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of ?-tubulin acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia. [BMB Reports 2019; 52(10): 619-624].

SUBMITTER: Kim BH

PROVIDER: S-EPMC6827577 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Deficiency of calpain-6 inhibits primary ciliogenesis.

Kim Bo Hye BH Kim Do Yeon DY Oh Sumin S Ko Je Yeong JY Rah Gyuyeong G Yoo Kyung Hyun KH Park Jong Hoon JH

BMB reports 20191001 10

The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding hum ...[more]

PMID: 31619317

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