Dataset Information

Enhanced Microbial Interactions and Deterministic Successions During Anoxic Decomposition of Microcystis Biomass in Lake Sediment.

ABSTRACT: Microcystis biomass remineralization after blooming represents a hotspot of nutrient recycling in eutrophic lakes. Because Microcystis blooms are massively deposited on lake sediments, resulting in anoxic conditions, it is important to understand the response and role of benthic microbial communities during the anoxic decomposition of Microcystis in freshwater lakes. In the present study, we employed a microcosm method, combined with high-throughput sequencing, functional prediction, and network analysis, to investigate microbial succession during the short-term (30 days) anaerobic decomposition of Microcystis in a eutrophic sediment. Continuous accumulation of CH4 and CO2 and increasing relative abundance of methanogens were observed during the incubation. The microbial community composition (MCC) significantly changed after addition of Microcystis biomass, with a shift in the community from a stochastic to a functional, deterministic succession. Families, including Clostridiaceae, Rhodocyclaceae, Rikenellaceae, Peptostreptococcaceae, Syntrophomonadaceae, Lachnospiraceae, and Methanosarcinaceae, were predominantly enriched and formed diverse substitution patterns, suggesting a synergistic action of these family members in the decomposition of Microcystis biomass. Importantly, intense species-to-species interactions and weak resistance to disturbance were observed in the microbial community after Microcystis biomass addition. Collectively, these results suggest that the addition of Microcystis induce phylogenetic clustering and structure instability in the sediment microbial community and the synergistic interactions among saprotrophic bacteria play a key role in Microcystis biomass remineralization.

SUBMITTER: Wu YF

PROVIDER: S-EPMC6831559 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Enhanced Microbial Interactions and Deterministic Successions During Anoxic Decomposition of Microcystis Biomass in Lake Sediment.

Wu Yu-Fan YF Xing Peng P Liu Shuangjiang S Wu Qinglong L QL

Frontiers in microbiology 20191030

Microcystis biomass remineralization after blooming represents a hotspot of nutrient recycling in eutrophic lakes. Because Microcystis blooms are massively deposited on lake sediments, resulting in anoxic conditions, it is important to understand the response and role of benthic microbial communities during the anoxic decomposition of Microcystis in freshwater lakes. In the present study, we employed a microcosm method, combined with high-throughput sequencing, functional pr ...[more]

PMID: 31736913

Similar Datasets

Project description:The current study reports the community succession of different toxin and non-toxin producing cyanobacteria at different stages of cyanobacterial harmful algal blooms (CyanoHABs) and their connectivity with nitrogen and phosphorus cycles in a freshwater lake using an ecogenomics framework. Comprehensive high throughput DNA sequencing, water quality parameter measurements, and functional gene expressions over temporal and spatial scales were employed. Among the cyanobacterial community, the lake was initially dominated by Cyanobium during the months of May, June, and early July, and later primarily by Aphanizomenon and Dolichospermum depicting functional redundancy. Finally, Planktothrix appeared in late August and then the dominance switched to Planktothrix in September. Microcystis aeruginosa and Microcystis panniformis; two species responsible for cyanotoxin production, were also present in August and September, but in significantly smaller relative abundance. MC-LR (0.06-1.32 µg/L) and MC-RR (0.01-0.26 µg/L) were two major types of cyanotoxins detected. The presence of MC-LR and MC-RR were significantly correlated with the Microcystis-related genes (16SMic/mcyA/mcyG) and their expressions (r = 0.33 to 0.8, p < 0.05). The metabolic analyses further linked the presence of different cyanobacterial groups with distinct functions. The nitrogen metabolisms detected a relatively higher abundance of nitrite/nitrate reductase in early summer, indicating significant denitrification activity and the activation of N-fixation in the blooms dominated by Aphanizomenon/Dolichospermum (community richness) during nutrient-limited conditions. The phosphorus and carbohydrate metabolisms detected a trend to initiate a nutrient starvation alert and store nutrients from early summer, while utilizing the stored polyphosphate and carbohydrate (PPX and F6PPK) during the extreme ortho-P scarcity period, mostly in August or September. Specifically, the abundance of Aphanizomenon and Dolichospermum was positively correlated with the nitrogen-fixing nif gene and (p < 0.001) and the PPX enzyme for the stored polyphosphate utilization (r = 0.77, p < 0.001). Interestingly, the lake experienced a longer N-fixing period (2-3 months) before non-fixing cyanobacteria (Planktothrix) dominated the entire lake in late summer. The Provo Bay site, which is known to be nutrient-rich historically, had early episodes of filamentous cyanobacteria blooms compared to the rest of the lake.

Project description:Active sulfate-reducing microorganisms (SRM) in freshwater sediments are under-examined, despite the well-documented cryptic sulfur cycle occurring in these low-sulfate habitats. In Lake Constance sediment, sulfate reduction rates of up to 1,800 nmol cm-3 day-1 were previously measured. To characterize its SRM community, we used a tripartite amplicon sequencing approach based on 16S rRNA genes, 16S rRNA, and dsrB transcripts (encoding the beta subunit of dissimilatory sulfite reductase). We followed the respective amplicon dynamics in four anoxic microcosm setups supplemented either with (i) chitin and sulfate, (ii) sulfate only, (iii) chitin only, or (iv) no amendment. Chitin was used as a general substrate for the whole carbon degradation chain. Sulfate turnover in sulfate-supplemented microcosms ranged from 38 to 955 nmol day-1 (g sediment f. wt.)-1 and was paralleled by a decrease of 90-100% in methanogenesis as compared to the respective methanogenic controls. In the initial sediment, relative abundances of recognized SRM lineages accounted for 3.1 and 4.4% of all bacterial 16S rRNA gene and 16S rRNA sequences, respectively. When normalized against the 1.4 × 108 total prokaryotic 16S rRNA gene copies as determined by qPCR and taking multiple rrn operons per genome into account, this resulted in approximately 105-106 SRM cells (g sediment f. wt.)-1. The three amplicon approaches jointly identified Desulfobacteraceae and Syntrophobacteraceae as the numerically dominant and transcriptionally most active SRM in the initial sediment. This was corroborated in the time course analyses of sulfate-consuming sediment microcosms irrespective of chitin amendment. Uncultured dsrAB family-level lineages constituted in sum only 1.9% of all dsrB transcripts, with uncultured lineage 5 and 6 being transcriptionally most active. Our study is the first holistic molecular approach to quantify and characterize active SRM including uncultured dsrAB lineages not only in Lake Constance but for lake sediments in general.

Dataset Information

Enhanced Microbial Interactions and Deterministic Successions During Anoxic Decomposition of Microcystis Biomass in Lake Sediment.

Publications

Enhanced Microbial Interactions and Deterministic Successions During Anoxic Decomposition of <i>Microcystis</i> Biomass in Lake Sediment.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets