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Analysis of high-throughput sequencing for cecal microbiota diversity and function in hens under different rearing systems.


ABSTRACT: Rearing systems play an important role in animal welfare, health and the composition of the gut microbiome. Therefore, the purpose of this study was to investigate the effects of different rearing systems on the composition and function of cecal microbiota in chickens. The 120-day-old Lohmann hens of cage rearing systems (CRS) and free-range systems (FRS) were studied. The cecal bacterial populations of hens were surveyed by high-throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3-V4 combined with metagenomic sequencing analysis. The 16S rRNA sequencing analysis showed that the cecal microbiota differed between the FRS and CRS. The three most abundant bacteria phyla in the two systems were the Bacteroidetes (>?48%), Firmicutes (>?37%), and Proteobacteria (>?6%), the Deferribacteres (>?2.4%) were found in FRS and almost absent in CRS (Bacteroides, Rikenellaceae_RC9, and Faecalibacterium, and we found relative abundance of the Parabacteroides (P?Prevotellaceae_Ga6A1 (P?Proteobacteria (P?Spirochaetaceae (P?Faecalibacterium, Ruminococcaceae, and Helicobacter was greater in CRS (P?

SUBMITTER: Shi S 

PROVIDER: S-EPMC6834809 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Analysis of high-throughput sequencing for cecal microbiota diversity and function in hens under different rearing systems.

Shi Shuiqin S   Qi Zhao Z   Gu Bintao B   Cheng Baoyan B   Tu Jian J   Song Xiangjun X   Shao Yin Y   Liu Hongmei H   Qi Kezong K   Li Shaowen S  

3 Biotech 20191106 12


Rearing systems play an important role in animal welfare, health and the composition of the gut microbiome. Therefore, the purpose of this study was to investigate the effects of different rearing systems on the composition and function of cecal microbiota in chickens. The 120-day-old Lohmann hens of cage rearing systems (CRS) and free-range systems (FRS) were studied. The cecal bacterial populations of hens were surveyed by high-throughput sequencing (HTS) of the bacterial 16S rRNA hypervariabl  ...[more]

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