In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.

ABSTRACT: The study of exosomes has become increasingly popular due to their potentially important biological roles. Urine can be used as an effective source of exosomes for noninvasive investigations into the pathophysiological states of the urinary system, but first, detailed characterization of exosomal components in healthy individuals is essential. Here, we significantly extend the number of N-glycan compositions, including sulfated species, identified from urinary exosomes and determine the sialic acid linkages for many of those compositions. Capillary electrophoresis-mass spectrometry (CE-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), and capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify N-glycan and sulfated N-glycan com

SUBMITTER: Song W 

PROVIDER: S-EPMC6834888 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature