Direct fluorescent glycan labeling with recombinant sialyltransferases.
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ABSTRACT:
SUBMITTER: Wu ZL
PROVIDER: S-EPMC6835046 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Publications
Direct fluorescent glycan labeling with recombinant sialyltransferases.
Wu Zhengliang L ZL Person Anthony D AD Burton Andrew J AJ Singh Ravinder R Burroughs Barbara B Fryxell Dan D Tatge Timothy J TJ Manning Timothy T Wu Guoping G Swift Karl A D KAD Kalabokis Vassili V
Glycobiology 20191001 11
Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blot ...[more]
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