A Robust DNA Isolation Protocol from Filtered Commercial Olive Oil for PCR-Based Fingerprinting.

ABSTRACT: Extra virgin olive oil (EVOO) has elevated commercial value due to its health appeal, desirable characteristics and quantitatively limited production, and thus it has become an object of intentional adulteration. As EVOOs on the market might consist of a blend of olive varieties or sometimes even of a mixture of oils from different botanical species, an array of DNA-fingerprinting methods have been developed to check the varietal composition of the blend. Starting from a comparison between publicly available DNA extraction protocols, we set up a timely, low-cost, reproducible and effective DNA isolation protocol, which allows an adequate amount of DNA to be recovered even from commercial filtered EVOOs. Then, in order to verify the effectiveness of the DNA extraction protocol herein propos

SUBMITTER: Piarulli L 

PROVIDER: S-EPMC6836273 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature