Ontology highlight
ABSTRACT:
SUBMITTER: Foley JW
PROVIDER: S-EPMC6836736 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Foley Joseph W JW Zhu Chunfang C Jolivet Philippe P Zhu Shirley X SX Lu Peipei P Meaney Michael J MJ West Robert B RB
Genome research 20190913 11
RNA sequencing (RNA-seq) is a sensitive and accurate method for quantifying gene expression. Small samples or those whose RNA is degraded, such as formalin-fixed paraffin-embedded (FFPE) tissue, remain challenging to study with nonspecialized RNA-seq protocols. Here, we present a new method, Smart-3SEQ, that accurately quantifies transcript abundance even with small amounts of total RNA and effectively characterizes small samples extracted by laser-capture microdissection (LCM) from FFPE tissue. ...[more]