Dataset Information

Impact of UVC-sustained recirculating air filtration on airborne bacteria and dust in a pig facility.

ABSTRACT: High amounts of aerial pollutants like dust and microorganisms can pose serious health hazards to animals and humans. The aim of the current study therefore was, to assess the efficiency of UVC irradiation combined to air filtration in reducing airborne microorganisms at laboratory scale. In a second part, a UVC-combined recirculating air filtration module (UVC module) was implemented in a small animal facility in order to assess its improvement of air quality with regard to airborne bacteria and dust. Tests at laboratory scale were performed using aerosols of Staphylococcus (S.) aureus, Actinobacillus pleuropneumoniae, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus. We varied relative humidity (RH) to evaluate its effect on UVC irradiation efficiency. In addition, viability of pathogens inside the filter material was determined over up to six months. UVC-combined air filtration resulted in a more than 99% reduction of viral and bacterial particles. RH had no influence on UVC efficiency. Viability in the filter matter varied depending on the pathogen used and RH with S. aureus and PPV being most resistant. In our small pig facility consisting of two separated barns, weekly air measurements were conducted over a period of 13 weeks (10 piglets) and 16 weeks (11 piglets), respectively. Airborne bacterial numbers were significantly lower in the barn equipped with the UVC module compared to the reference barn. On average a reduction to 37% of reference values could be achieved for bacteria, whereas the amount of total dust was reduced to a much lesser extent (i.e. to 78% of reference values). Measures taken in front of and behind the UVC module revealed a reduction of 99.4% for airborne bacteria and 95.0% for total dust. To conclude, recirculating air filtration combined to UVC provided efficient reduction of pathogens at laboratory and experimental scale. The implementation of such devices might improve the overall environmental quality in animal facilities.

SUBMITTER: Eisenloffel L

PROVIDER: S-EPMC6837447 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Impact of UVC-sustained recirculating air filtration on airborne bacteria and dust in a pig facility.

Eisenlöffel Lisa L Reutter Tobias T Horn Matthias M Schlegel Simon S Truyen Uwe U Speck Stephanie S

PloS one 20191107 11

High amounts of aerial pollutants like dust and microorganisms can pose serious health hazards to animals and humans. The aim of the current study therefore was, to assess the efficiency of UVC irradiation combined to air filtration in reducing airborne microorganisms at laboratory scale. In a second part, a UVC-combined recirculating air filtration module (UVC module) was implemented in a small animal facility in order to assess its improvement of air quality with regard to airborne bacteria an ...[more]

PMID: 31697778

Similar Datasets

Project description:The COVID-19 pandemic has revealed the importance of the detection of airborne pathogens. Here, we present composite air filters featuring a bioinspired liquid coating that facilitates the removal of captured aerosolized bacteria and viruses for further analysis. We tested three types of air filters: commercial polytetrafluoroethylene (PTFE), which is well known for creating stable liquid coatings, commercial high-efficiency particulate air (HEPA) filters, which are widely used, and in-house-manufactured cellulose nanofiber mats (CNFMs), which are made from sustainable materials. All filters were coated with omniphobic fluorinated liquid to maximize the release of pathogens. We found that coating both the PTFE and HEPA filters with liquid improved the rate at which Escherichia coli was recovered using a physical removal process compared to uncoated controls. Notably, the coated HEPA filters also increased the total number of recovered cells by 57%. Coating the CNFM filters did not improve either the rate of release or the total number of captured cells. The most promising materials, the liquid-coated HEPA, filters were then evaluated for their ability to facilitate the removal of pathogenic viruses via a chemical removal process. Recovery of infectious JC polyomavirus, a nonenveloped virus that attacks the central nervous system, was increased by 92% over uncoated controls; however, there was no significant difference in the total amount of genomic material recovered compared to that of controls. In contrast, significantly more genomic material was recovered for SARS-CoV-2, the airborne, enveloped virus, which causes COVID-19, from liquid-coated filters. Although the amount of infectious SARS-CoV-2 recovered was 58% higher, these results were not significantly different from uncoated filters due to high variability. These results suggest that the efficient recovery of airborne pathogens from liquid-coated filters could improve air sampling efforts, enhancing biosurveillance and global pathogen early warning.

Project description:Settled airborne dust is used as a surrogate for airborne exposure in studies that explore indoor microbes. In order to determine whether detecting differences in dust environments would depend on the sampler type, we compared different passive, settled dust sampling approaches with respect to displaying qualitative and quantitative aspects of the bacterial and fungal indoor microbiota.Settled dust sampling approaches-utilizing plastic petri dishes, TefTex material, and electrostatic dustfall collectors (EDCs)-were evaluated in indoor spaces in the USA and Finland and in an experimental chamber study. The microbial content was analyzed with quantitative PCR (qPCR) to quantify total bacterial and fungal biomass and through high-throughput sequencing to examine bacterial community composition. Bacterial composition and diversity were similar within a sampling environment regardless of the sampler type. The sampling environment was the single largest predictor of microbial community composition within a study, while sampler type was found to have much less predictive power. Quantitative analyses in indoor spaces indicated highest yields using a petri dish approach, followed by sampling with EDCs and TefTex. The highest correlations between duplicate samples were observed for EDC and petri dish approaches, indicating greater experimental repeatability for these sampler types. For the EDC samples, it became apparent that, due to the fibrous nature of the material, a rigorous extraction protocol is crucial to obtain optimal yields and stable, repeatable results.Correlations between sampler types were strong both in compositional and quantitative terms, and thus, the particular choice of passive settled dust sampler is not likely to strongly alter the overall conclusion of a study that aims to characterize dust across different environments. Microbial cell abundances determined from settled dust varied with the use of different sampling approaches, and thus, consistency in the method is necessary to allow for absolute comparisons within and among studies. Considering practical aspects, petri dishes were found to be an inexpensive, simple, and feasible approach that showed the highest quantitative determinations under typical building conditions, though the choice of sampler will ultimately depend on study logistics and characteristics such as low- or high-exposure settings.

Dataset Information

Impact of UVC-sustained recirculating air filtration on airborne bacteria and dust in a pig facility.

Publications

Impact of UVC-sustained recirculating air filtration on airborne bacteria and dust in a pig facility.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets