Dataset Information

In vitro and in vivo datasets of topically applied ketorolac tromethamine in aqueous humor using Raman spectroscopy.

ABSTRACT: This article includes datasets acquired by Raman spectroscopy from in vivo and in vitro ocular samples collected from the dataset from Bertens and Zhang et al., "Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo" (Bertens and Zhang, et al.). Detection of ketorolac tromethamine in pig eyes was performed in vitro and rabbit eyes in vivo. Extracted aqueous humor samples from pig and rabbit eyes were measured in vitro using a cuvette. This manuscript shows the spectral Raman data without pre-treatment or analysis from ocular tissues and provides further information towards aqueous humor research via alternative data processing methods. Furthermore, the raw data enclosed may be used for future aqueous humor investigations and pharmaceutical research.

SUBMITTER: Zhang S

PROVIDER: S-EPMC6839021 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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In vitro and in vivo datasets of topically applied ketorolac tromethamine in aqueous humor using Raman spectroscopy.

Zhang Shuo S Bertens Christian J F CJF Erckens Roel J RJ van den Biggelaar Frank J H M FJHM Berendschot Tos T J M TTJM Webers Carroll A B CAB Nuijts Rudy M M A RMMA Gijs Marlies M

Data in brief 20191022

This article includes datasets acquired by Raman spectroscopy from in vivo and in vitro ocular samples collected from the dataset from Bertens and Zhang et al., "Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo" (Bertens and Zhang, et al.). Detection of ketorolac tromethamine in pig eyes was performed in vitro and rabbit eyes in vivo ...[more]

PMID: 31720341

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Project description:BackgroundBiochemical cervical change during labor is not well understood, in part, because of a dearth of technologies capable of safely probing the pregnant cervix in vivo. The need for such a technology is 2-fold: (1) to gain a mechanistic understanding of the cervical ripening and dilation process and (2) to provide an objective method for evaluating the cervical state to guide clinical decision-making. Raman spectroscopy demonstrates the potential to meet this need, as it is a noninvasive optical technique that can sensitively detect alterations in tissue components, such as extracellular matrix proteins, lipids, nucleic acids, and blood, which have been previously established to change during the cervical remodeling process.ObjectiveWe sought to demonstrate that Raman spectroscopy can longitudinally monitor biochemical changes in the laboring cervix to identify spectral markers of impending parturition.Study designOverall, 30 pregnant participants undergoing either spontaneous or induced labor were recruited. The Raman spectra were acquired in vivo at 4-hour intervals throughout labor until rupture of membranes using a Raman system with a fiber-optic probe. Linear mixed-effects models were used to determine significant (P<.05) changes in peak intensities or peak ratios as a function of time to delivery in the study population. A nonnegative least-squares biochemical model was used to extract the changing contributions of specific molecule classes over time.ResultsWe detected multiple biochemical changes during labor, including (1) significant decreases in Raman spectral features associated with collagen and other extracellular matrix proteins (P=.0054) attributed to collagen dispersion, (2) an increase in spectral features associated with blood (P=.0372), and (3) an increase in features indicative of lipid-based molecules (P=.0273). The nonnegative least-squares model revealed a decrease in collagen contribution with time to delivery, an increase in blood contribution, and a change in lipid contribution.ConclusionOur findings have demonstrated that in vivo Raman spectroscopy is sensitive to multiple biochemical remodeling changes in the cervix during labor. Furthermore, in vivo Raman spectroscopy may be a valuable noninvasive tool for objectively evaluating the cervix to potentially guide clinical management of labor.

Dataset Information

In vitro and in vivo datasets of topically applied ketorolac tromethamine in aqueous humor using Raman spectroscopy.

Publications

<i>In vitro</i> and <i>in vivo</i> datasets of topically applied ketorolac tromethamine in aqueous humor using Raman spectroscopy.

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