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Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno-Associated Virus.

ABSTRACT: Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV-based method can facilitate gene function studies in early-stage embryos.

SUBMITTER: Wang D

PROVIDER: S-EPMC6839749 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno-Associated Virus.

Wang Dan D Niu Yuyu Y Ren Lingzhi L Kang Yu Y Tai Phillip W L PWL Si Chenyang C Mendonca Craig A CA Ma Hong H Gao Guangping G Ji Weizhi W

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20190904 21

Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno-associated viruses (rAAVs). Together with previous reports from the authors of this paper and ...[more]

PMID: 31728271

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