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Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar.

ABSTRACT: Freezing tolerant tea cultivars are urgently needed. The tea cultivars with highly freezing tolerance showed resistance to freezing stress induced photoinhibition. Freezing sensitivity index (H) of 47 tea clonal cultivars was investigated after severe freezing winter in 2016. To develop instrumental methods for freezing tolerance selection, the maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm) and leaf color indicator a on the Hunter color scale were determined on control group (non-frozen) and frozen group (being frozen at -15 °C for 2 h and then stood at 20 °C for 5 h) of the cultivars. When the two indicators were expressed as the ratios (RFv/Fm and Ra) of frozen group to control group, linear regression of the freezing sensitivity index (H) upon the RFv/Fm and Ra produced significant relationship respectively, i.e., H = 60.31 - 50.09 RFv/Fm (p < 0.01) and H = 30.03 - 10.82 Ra (p < 0.01). Expression of gene psbA encoding D1 protein and gene psbD encoding D2 protein in PSII showed that the frezzing tolerant tea cultivars maintained a high expression level of psbA after freezing stress, which is considered to be beneficial to de novo synthesis of D1 protein and sustaining PSII activity. These findings can provide instrumental tools for assessing freezing tolerance of tea cultivars in tea breeding program.

SUBMITTER: Shi Y

PROVIDER: S-EPMC6843692 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar.

Shi Yunlong Y Cai Zhuoyu Z Li Da D Lu Jianliang J Ye Jianhui J Liang Yuerong Y Zheng Xinqiang X

Plants (Basel, Switzerland) 20191022 10

Freezing tolerant tea cultivars are urgently needed. The tea cultivars with highly freezing tolerance showed resistance to freezing stress induced photoinhibition. Freezing sensitivity index (H) of 47 tea clonal cultivars was investigated after severe freezing winter in 2016. To develop instrumental methods for freezing tolerance selection, the maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm) and leaf color indicator <i>a</i> on the Hunter color scale were determined on control ...[more]

PMID: 31652528

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Project description:Chickpea (Cicer arietinum) is a legume of the family Fabaceae, subfamily Faboideae. In Egypt, chickpea seeds are usually consumed at raw green and tender stage, or in the form of mature dry seeds. In our previous study, 'Giza 1' seeds exhibited stronger antioxidant activity and higher total phenol content than those from other Egyptian cultivars. In order to assess the biological potential of 'Giza 1' seeds in vivo, the extraction procedure was reproduced here. The extract was standardized using liquid chromatography coupled to diode array detector and tandem mass spectrometry (MS/MS) to evaluate their hepatoprotective effect on carbon tetrachloride (CCl4)-induced hepatotoxicity in rats and acute toxicity. Administration of the extract to rats in doses up to 2 g/Kg) did not cause any mortalities or observable signs of toxicity. Further, the plant extract showed a strong hepatoprotective activity based on assessing serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and levels of albumen, globulin, total protein, total cholesterol, high density lipoprotein, triglycerides, and low density lipoprotein. The antioxidative activity was evaluated by assessing hepatic catalase and superoxide dismutase activity as well as reduced glutathione, and malondialdehyde levels. Additionally, anti-inflammatory activity was observed as the extract significantly lowered the hepatic tumor necrosis factor ? content. Histopathological examination of liver tissues indicated that the extract-treated animals showed almost normal hepatic architecture with fewer pathological changes. In conclusion, the current results suggest that the chickpea extract possesses an excellent safety profile with very low acute toxicity. Also, it exhibits a significant hepatoprotective effect against CCl4-induced liver injury in rats. This can be attributed, at least partly, to the antioxidant and anti-inflammatory activity of the isoflavones and phenolic acids content of the extract.

Dataset Information

Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar.

Publications

Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar.

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