Ontology highlight
ABSTRACT:
SUBMITTER: Mao S
PROVIDER: S-EPMC6847002 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Mao Shiqi S Ying Yachen Y Wu Xiaotian X Krueger Christopher J CJ Chen Antony K AK
Nucleic acids research 20191101 20
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genom ...[more]