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CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.

ABSTRACT: Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genomic loci with only three unique sgRNAs.

SUBMITTER: Mao S

PROVIDER: S-EPMC6847002 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci.

Mao Shiqi S Ying Yachen Y Wu Xiaotian X Krueger Christopher J CJ Chen Antony K AK

Nucleic acids research 20191101 20

Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genom ...[more]

PMID: 31504824

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