Ontology highlight
ABSTRACT:
SUBMITTER: Matveeva E
PROVIDER: S-EPMC6848856 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Matveeva Evgenia E Gryczynski Zygmunt Z Gryczynski Ignacy I Malicka Joanna J Lakowicz Joseph R JR
Analytical chemistry 20041101 21
We described an immunoassay for the cardiac marker myoglobin on a thin silver mirror surface using surface plasmon-coupled emission (SPCE). SPCE occurs for fluorophores in proximity (within approximately 200 nm) of a thin metal film (in our case, silver) and results in a highly directional radiation through a glass substrate at a well-defined angle from the normal axis. We used the effect of SPCE to develop a myoglobin immunoassay on the silver mirror surface deposited on a glass substrate. Bind ...[more]