Calpastatin participates in the regulation of cell migration in BAP1-deficient uveal melanoma cells.
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ABSTRACT:
SUBMITTER: Yue H
PROVIDER: S-EPMC6848861 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Calpastatin participates in the regulation of cell migration in BAP1-deficient uveal melanoma cells.
Yue Han H Meng Feng-Xi FX Qian Jiang J Xu Bin-Bin BB Li Gang G Wu Ji-Hong JH
International journal of ophthalmology 20191118 11
<h4>Aim</h4>To detect how BRCA-associated protein 1 (BAP1) regulates cell migration in uveal melanoma (UM) cells.<h4>Methods</h4>Wound healing and transwell assays were performed to detect UM cell migration abilities. Protein chip, immunoprecipitations and surface plasmon resonance analyses were applied to identify BAP1 protein partners. Western blot and calpain activity assays were used to test the expression and function of calpastatin (CAST).<h4>Results</h4>CAST protein was confirmed as a new ...[more]
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