Browse
Submit Data
Databases
API
Help

Dataset Information

21 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Strategies for complete plastid genome sequencing.

ABSTRACT: Plastid sequencing is an essential tool in the study of plant evolution. This high-copy organelle is one of the most technically accessible regions of the genome, and its sequence conservation makes it a valuable region for comparative genome evolution, phylogenetic analysis and population studies. Here, we discuss recent innovations and approaches for de novo plastid assembly that harness genomic tools. We focus on technical developments including low-cost sequence library preparation approaches for genome skimming, enrichment via hybrid baits and methylation-sensitive capture, sequence platforms with higher read outputs and longer read lengths, and automated tools for assembly. These developments allow for a much more streamlined assembly than via conventional short-range PCR. Although newer methods make complete plastid sequencing possible for any land plant or green alga, there are still challenges for producing finished plastomes particularly from herbarium material or from structurally divergent plastids such as those of parasitic plants.

SUBMITTER: Twyford AD

PROVIDER: S-EPMC6849563 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Strategies for complete plastid genome sequencing.

Twyford Alex D AD Ness Rob W RW

Molecular ecology resources 20161128 5

Plastid sequencing is an essential tool in the study of plant evolution. This high-copy organelle is one of the most technically accessible regions of the genome, and its sequence conservation makes it a valuable region for comparative genome evolution, phylogenetic analysis and population studies. Here, we discuss recent innovations and approaches for de novo plastid assembly that harness genomic tools. We focus on technical developments including low-cost sequence library preparation approache ...[more]

PMID: 27790830

Similar Datasets

Complete Plastid Genome Sequencing of Four Tilia Species (Malvaceae): A Comparative Analysis and Phylogenetic Implications.

Project description:Tilia is an ecologically and economically important genus in the family Malvaceae. However, there is no complete plastid genome of Tilia sequenced to date, and the taxonomy of Tilia is difficult owing to frequent hybridization and polyploidization. A well-supported interspecific relationships of this genus is not available due to limited informative sites from the commonly used molecular markers. We report here the complete plastid genome sequences of four Tilia species determined by the Illumina technology. The Tilia plastid genome is 162,653 bp to 162,796 bp in length, encoding 113 unique genes and a total number of 130 genes. The gene order and organization of the Tilia plastid genome exhibits the general structure of angiosperms and is very similar to other published plastid genomes of Malvaceae. As other long-lived tree genera, the sequence divergence among the four Tilia plastid genomes is very low. And we analyzed the nucleotide substitution patterns and the evolution of insertions and deletions in the Tilia plastid genomes. Finally, we build a phylogeny of the four sampled Tilia species with high supports using plastid phylogenomics, suggesting that it is an efficient way to resolve the phylogenetic relationships of this genus.

| S-EPMC4643919 | biostudies-literature

A strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing.

Project description:PREMISE OF THE STUDY:We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. METHODS:A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. RESULTS:From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs. This weedy rice was classified based on 23 polymorphic regions identified by comparison with reference chloroplast genomes. We detected recent and past events of genetic material transfer between the chloroplast and nuclear genomes and estimated their occurrence frequency. DISCUSSION:We obtained a high-quality complete chloroplast genome sequence from low-coverage sequencing data. Intergenome DNA transfer appears to be more frequent than previously thought.

| S-EPMC4610308 | biostudies-literature

Complete plastid genome of Dendrobium naungmungense (Orchidaceae).

Project description:Dendrobium is one of the most important genera in Orchidaceae. In this study, we used the next-generation sequencing technology and assembled a complete plastid genome of a recently published new species of Dendrobium, D. naungmungense. The plastome was 151,883?bp in length, containing a large single-copy region (LSC) of 87,189?bp, and a small single-copy region (SSC) of 12,150?bp, and two inverted repeat regions (IR) of 26,272?bp. A total of 123 genes were predicted, including 38 tRNA genes, 8 rRNA genes, and 77 protein-coding genes. Phylogenetic analysis of 44 representative plastome of the genus Dendrobium and outgroup suggested D. naungmungense to be sister to Dendrobium wardianum.

| S-EPMC7707356 | biostudies-literature

Characterization of the complete plastid genome of Butia eriospatha (Arecaceae).

Project description:Butia eriospatha is an endemic palm species from the Atlantic Rainforest in Brazil, a biodiversity hotspot. This species is currently listed in the IUCN red list as vulnerable and lacks specific plastid markers for population genetics studies. In addition, the evolutionary relationship within the genus Butia is not yet well resolved. Here, we sequenced and characterized the complete plastid genome (plastome) sequence of B. eriospatha. The complete plastome sequence is 154,048 bp in length, with the typical quadripartite structure. This plastome length and genes content is consistent with other six species from tribe Cocoseae. However, the Inverted Repeat (IR) borders show some variation among the analyzed species from this tribe. Species from the Bactridinae (Astrocaryum and Acrocomia) and Elaeidinae (Elaeis) subtribes present the rps19 gene completely duplicated in the IR region. In contrast, all plastomes sequenced from the subtribe Attaleinae (Butia, Cocos, Syagrus) present one complete CDS of rps19 and one partial copy of rps19. The difference in the IR/LSC junctions between Attaleinae and the sister clades Bactridinae + Elaeidinae might be considered an evolutionary signal and the plastome sequence of B. eriopatha may be used in future studies of population genetics and phylogeny.

| S-EPMC7488953 | biostudies-literature

The complete plastid genome sequence of Begonia guangxiensis.

Project description:Begonia guangxiensis was assessed as endangered according to Red List of Chinese Plants. In this study, we described the complete plastid genome of B. guangxiensis. The plastid genome sequence of B. guangxiensis is 157,648?bp in size, having a large single-copy region (LSC) of 86,514?bp, a small single-copy region (SSC) of 18,076?bp, and two inverted repeat (IR) regions of 26,529?bp. The complete plastid genome of B. guangxiensis encoding 112 unique genes including 79 protein-coding genes, 29 tRNA genes, and four rRNA genes. The overall GC content of the plastid genome is 35.9%. Phylogenetic analysis confirmed that B. guangxiensis is closely related to B. varipeltata.

| S-EPMC7707394 | biostudies-literature

Complete plastid genome of Cheirostylis chinensis (Orchidaceae, Goodyerinae).

Project description:The first complete plastid genome of Cheirostylis, Ch. chinensis, was assembled and analyzed in this study. The total genome was 147,218 bp in length, consisting of a large single-copy region (LSC) of 81,081 bp, a small single-copy region (SSC) of 14,769 bp, and two inverted repeat regions (IRA and IRB) of 25,684 bp. The genome contained 131 genes, including 38 transfer RNA (tRNA) genes, 8 ribosomal RNA (rRNA) genes and 85 protein-coding genes. Phylogenomic analysis indicated that Ch. chinensis nested within Goodyerinae.

| S-EPMC7720991 | biostudies-literature

The complete plastid genome of Ficus erecta (Moraceae).

Project description:Ficus erecta is a wild relative of F. carica, which is an important economic plant. Here, we determined the complete plastid genome of F. erecta using the Illumina paired reads to provide genomic feature resources. The whole plastid genome of F. erecta is 160,603 bp in length, containing two inverted repeats (IRs) of 25,899 bp separated by a large single-copy (LSC 88,640 bp) region and a small single-copy (SSC 20,165 bp) region. The complete plastome sequence of Ficus erecta will provide a useful resource for the evolutionary biology study as well as for the phylogenetic studies.

| S-EPMC7783041 | biostudies-literature

Complete plastid genome of Cumathamnion serrulatum (Ceramiales, Rhodophyta).

Project description:We report the complete plastid genome of Cumathamnion serrulatum, also known as Delesseria serrulata. The plastid genome was 174,192 bp in size. Annotation showed there were 193 protein coding genes, three ribosomal RNAs, and 29 transfer RNAs. One intron was found, and the GC content was 27.2%. The maximum likelihood tree with the concatenated 177 plastid coding genes showed a strong monophyletic relationship to Membranoptera spp. within the Ceramiales.

| S-EPMC8208111 | biostudies-literature

Complete Plastid Genome Sequence of the Brown Alga Undaria pinnatifida.

Project description:In this study, we fully sequenced the circular plastid genome of a brown alga, Undaria pinnatifida. The genome is 130,383 base pairs (bp) in size; it contains a large single-copy (LSC, 76,598 bp) and a small single-copy region (SSC, 42,977 bp), separated by two inverted repeats (IRa and IRb: 5,404 bp). The genome contains 139 protein-coding, 28 tRNA, and 6 rRNA genes; none of these genes contains introns. Organization and gene contents of the U. pinnatifida plastid genome were similar to those of Saccharina japonica. There is a co-linear relationship between the plastid genome of U. pinnatifida and that of three previously sequenced large brown algal species. Phylogenetic analyses of 43 taxa based on 23 plastid protein-coding genes grouped all plastids into a red or green lineage. In the large brown algae branch, U. pinnatifida and S. japonica formed a sister clade with much closer relationship to Ectocarpus siliculosus than to Fucus vesiculosus. For the first time, the start codon ATT was identified in the plastid genome of large brown algae, in the atpA gene of U. pinnatifida. In addition, we found a gene-length change induced by a 3-bp repetitive DNA in ycf35 and ilvB genes of the U. pinnatifida plastid genome.

| S-EPMC4591262 | biostudies-literature

Complete plastid genome of Vanda concolor (Orchidaceae, Aeridinae).

Project description:Vanda is one of the five most horticulturally important genera in Orchidaceae. In this study, we assembled the complete plastid genome of an important cultivated species, V. concolor. The plastome was 149,474 bp in length, containing a large single-copy region (LSC) of 85,678 bp, a small single-copy region (SSC) of 12,002 bp, and two inverted repeat regions (IR) of 25,897 bp. A total of 127 genes were predicted, including 38 tRNA, 8 rRNA, and 74 protein-coding genes. Phylogenetic analysis of 21 representative plastome of the subtribe Aeridinae suggested V. concolor to be sister to V. brunnea and that this pair is sister to Neofinetia.

| S-EPMC7707440 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data