Dataset Information
A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing.
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ABSTRACT: Background
?-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed that dominant expression of splicing variants in the Orange (Or) gene causes ?-carotene accumulation in cauliflower curd. In this study, we focused on a putative orthologue of the cauliflower or gene in rice, Osor, and attempt to accumulate ?-carotene in rice callus by modification of the Osor gene via genome editing using CRISPR/Cas9.Findings
CRISPR/Cas9 vectors for the Osor gene were constructed and transformed into rice calli. Some transformed calli showed orange color due to ?-carotene hyper-accumulation. Molecular analyses suggest that orange-colored calli are due to an abundance of in-frame aberrant Osor transcripts, whereas out-of-frame mutations were not associated with orange color.Conclusions
We demonstrate that directed gene modification of the Osor gene via CRISPR/Cas9-mediated genome editing results in ?-carotene fortification in rice calli. To date, golden rice, which accumulates ?-carotene in rice endosperm, has been developed by conventional transgenic approaches. Our results suggest an alternative approach to enhancing ?-carotene accumulation in crops.
SUBMITTER: Endo A
PROVIDER: S-EPMC6851270 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing.
Rice (New York, N.Y.) 20191112 1
<h4>Background</h4>β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed that dominant expression of splicing variants in the Orange (Or) gene causes β-carotene accumulation in cauliflower curd. In this study, we focused on a putative orthologue of the cauliflower or gene in rice, Osor, and attempt to accumulate β-carotene in rice callus by m ...[more]
