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Effects of mine tailings exposure on early life stages of atlantic cod.

ABSTRACT: In Norway, mine tailings waste can be deposited by coastal submarine dispersal. Mine tailings slurry includes fine particles <10 µm with elevated levels of metals (e.g., copper, iron) from residual mineral ore. Prolonged suspension of small particles in the water column may bring them into contact with locally spawned pelagic fish eggs, including Atlantic cod, Gadus morhua. Newly fertilized cod embryos were exposed to suspended mine tailings particles up to 3.2 mg/L in flow-through aquaria for a total of 21 d. Significantly more particles adhered to the surface of the chorion from the high treatment after 11-d exposure, and dissolved Cu concentrations increased in the water (up to 0.36 ± 0.06 µg/L). There was no adverse effect on embryo mortality but an 8% elevation in larval mortality. There were no differences with treatment on timing of hatching, embryo and larva morphometrics, abnormalities, or cardiac activity. There was a treatment-dependent up-regulation of stress marker genes (hspa8, cyp1c1) but no indication of metal-induced activation of metallothionien (mt gene transcription). Transcription markers for DNA and histone methyltransferases did show treatment-related up-regulation, indicative of altered methylation in larvae when developmental methylation patterns are determined, indicating some level of chronic toxicity that may have longer-term effects. Environ Toxicol Chem 2019;38:1446-1454. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

SUBMITTER: Reinardy HC

PROVIDER: S-EPMC6851963 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Effects of mine tailings exposure on early life stages of atlantic cod.

Reinardy Helena C HC Pedersen Kristine B KB Nahrgang Jasmine J Frantzen Marianne M

Environmental toxicology and chemistry 20190620 7

In Norway, mine tailings waste can be deposited by coastal submarine dispersal. Mine tailings slurry includes fine particles <10 µm with elevated levels of metals (e.g., copper, iron) from residual mineral ore. Prolonged suspension of small particles in the water column may bring them into contact with locally spawned pelagic fish eggs, including Atlantic cod, Gadus morhua. Newly fertilized cod embryos were exposed to suspended mine tailings particles up to 3.2 mg/L in flow-through aquaria for a ...[more]

PMID: 30901098

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Project description:BACKGROUND:Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile. RESULTS:We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive. CONCLUSIONS:Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

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Effects of mine tailings exposure on early life stages of atlantic cod.

Publications

Effects of mine tailings exposure on early life stages of atlantic cod.

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