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Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide.


ABSTRACT: Disialosyl globopentaosylceramide (DSGb5) is often expressed by renal cell carcinomas. To investigate properties of DSGb5, we have prepared its oligosaccharide moiety by chemically synthesizing Gb5 which was enzymatically sialylated using the mammalian sialyltransferases ST3Gal1 and ST6GalNAc5. Glycan microarray binding studies indicate that Siglec-7 does not recognize DSGb5, and preferentially binds Neu5Ac?(2,8)Neu5Ac containing glycans.

SUBMITTER: 't Hart IME 

PROVIDER: S-EPMC6852662 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Chemoenzymatic synthesis of the oligosaccharide moiety of the tumor-associated antigen disialosyl globopentaosylceramide.

't Hart Ingrid M E IME   Li Tiehai T   Wolfert Margreet A MA   Wang Shuo S   Moremen Kelley W KW   Boons Geert-Jan GJ  

Organic & biomolecular chemistry 20190801 31


Disialosyl globopentaosylceramide (DSGb5) is often expressed by renal cell carcinomas. To investigate properties of DSGb5, we have prepared its oligosaccharide moiety by chemically synthesizing Gb5 which was enzymatically sialylated using the mammalian sialyltransferases ST3Gal1 and ST6GalNAc5. Glycan microarray binding studies indicate that Siglec-7 does not recognize DSGb5, and preferentially binds Neu5Acα(2,8)Neu5Ac containing glycans. ...[more]

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