Dataset Information

Hypomethylation in the promoter region of ZPBP as a potential litter size indicator in Berkshire pigs.

ABSTRACT: In pigs, litter size is typically defined as the total number of piglets born (TNB) or the number of piglets born alive (NBA). Increasing pig litter size is of great economic interest as a means to increase productivity. The capacity of the uterus is a critical component of litter size and may play a central role in prolificacy. In this study, we investigated litter-size-related epigenetic markers in uterine tissue from Berkshire pigs with smaller litter size groups (SLGs) and larger litter size groups (LLGs) using genome-wide bisulfite sequencing (GWBS). A total of 3269 differentially methylated regions (DMRs) were identified: 1566 were hypermethylated and 1703 hypomethylated in LLG compared to SLG. The zona pellucida binding protein (ZPBP) gene was significantly hypomethylated in the LLG promoter region, and its expression was significantly upregulated in uterine tissue. Thus, the methylation status of ZPBP gene was identified as a potential indicator of litter size. Furthermore, we verified its negative correlation with litter size traits (TNB and NBA) in whole blood samples from 172 Berkshire sows as a blood-based biomarker by a porcine methylation-specific restriction enzyme polymerase chain reaction (PMP) assay. The results suggest that the methylation status of the ZPBP gene can serve as a valuable epigenetic biomarker for hyperprolific sows.

SUBMITTER: An SM

PROVIDER: S-EPMC6852858 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Hypomethylation in the promoter region of <i>ZPBP</i> as a potential litter size indicator in Berkshire pigs.

An Sang Mi SM Kwon Seulgi S Hwang Jung Hye JH Yu Go Eun GE Kang Deok Gyeong DG Park Da Hye DH Kim Tae Wan TW Park Hwa Chun HC Ha Jeongim J Kim Chul Wook CW

Archives animal breeding 20190225 1

In pigs, litter size is typically defined as the total number of piglets born (TNB) or the number of piglets born alive (NBA). Increasing pig litter size is of great economic interest as a means to increase productivity. The capacity of the uterus is a critical component of litter size and may play a central role in prolificacy. In this study, we investigated litter-size-related epigenetic markers in uterine tissue from Berkshire pigs with smaller litter size groups (SLGs) and larger litter size ...[more]

PMID: 31807615

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Project description:BackgroundThe quantitative genetics theory argues that inbreeding depression and heterosis are founded on the existence of directional dominance. However, most procedures for genomic selection that have included dominance effects assumed prior symmetrical distributions. To address this, two alternatives can be considered: (1) assume the mean of dominance effects different from zero, and (2) use skewed distributions for the regularization of dominance effects. The aim of this study was to compare these approaches using two pig datasets and to confirm the presence of directional dominance.ResultsFour alternative models were implemented in two datasets of pig litter size that consisted of 13,449 and 11,581 records from 3631 and 2612 sows genotyped with the Illumina PorcineSNP60 BeadChip. The models evaluated included (1) a model that does not consider directional dominance (Model SN), (2) a model with a covariate b for the average individual homozygosity (Model SC), (3) a model with a parameter ? that reflects asymmetry in the context of skewed Gaussian distributions (Model AN), and (4) a model that includes both b and ? (Model Full). The results of the analysis showed that posterior probabilities of a negative b or a positive ? under Models SC and AN were higher than 0.99, which indicate positive directional dominance. This was confirmed with the predictions of inbreeding depression under Models Full, SC and AN, that were higher than in the SN Model. In spite of differences in posterior estimates of variance components between models, comparison of models based on LogCPO and DIC indicated that Model SC provided the best fit for the two datasets analyzed.ConclusionsOur results confirmed the presence of positive directional dominance for pig litter size and suggested that it should be taken into account when dominance effects are included in genomic evaluation procedures. The consequences of ignoring directional dominance may affect predictions of breeding values and can lead to biased prediction of inbreeding depression and performance of potential mates. A model that assumes Gaussian dominance effects that are centered on a non-zero mean is recommended, at least for datasets with similar features to those analyzed here.

Dataset Information

Hypomethylation in the promoter region of ZPBP as a potential litter size indicator in Berkshire pigs.

Publications

Hypomethylation in the promoter region of <i>ZPBP</i> as a potential litter size indicator in Berkshire pigs.

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