The effect of polymorphism in the FADS2 gene on the fatty acid composition of bovine milk.

ABSTRACT: Polyunsaturated fatty acids (PUFAs) play a role in a wide variety of physiological processes. They are produced by a series of desaturation and elongation reactions. ? -6-desaturase is a membrane-bound enzyme that catalyzes the conversion of ? -linolenic acid (C18:3 n -3) and linoleic acid (C18:2 n -6) to stearidonic acid (18:4 n -3) and ? -linolenic acid (18:3 n -6). It is encoded by the FADS2 gene located on bovine chromosome 29. The aim of this study was to identify a single nucleotide polymorphism in the FADS2 gene and to determine possible associations with milk fatty acid composition in two breeds of dairy cattle, i.e., Jersey and Polish Holstein-Friesian. Direct DNA sequencing revealed the presence of an A-to-G substitution in intron 3 of the FADS2 gene (rs209202414). Both populations were genotyped with an appropriate PCR-RFLP assay. The following genotype distributions were observed: for Jerseys, AA? = ?0.24, AG? = ?0.63, and GG? = ?0.13; for Polish Holstein-Friesians, AA? = ?0.17, AG? = ?0.40, and GG? = ?0.43. In Jerseys, statistically significant relationships were found between the FASD2 genotypes and the following milk fatty acids: lauric ( P=0.0486 ), behenic ( P=0.0199 ), lignoceric ( P=0.0209 ), oleic ( P=0.0386 ), eicosatrienoic ( P=0.0113 ), and docosadienoic ( P=0.0181 ). In Polish Holstein-Friesian cows, significant associations were observed for erucic ( P=0.0460 ) and docosahexaenoic ( P=0.0469 ) acids. The study indicated the A-to-G substitution (rs209202414) in the bovine FADS2 gene as a potential genetic marker for fatty acid composition in cattle milk.

SUBMITTER: Proskura WS 

PROVIDER: S-EPMC6852874 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature