Ontology highlight
ABSTRACT:
SUBMITTER: Pintilie G
PROVIDER: S-EPMC6853598 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Pintilie Grigore G Chen Dong-Hua DH Tran Bich Ngoc BN Jakana Joanita J Wu Jinlu J Hew Choy Leong CL Chiu Wah W
Structure (London, England : 1993) 20190822 10
SGIV, or Singapore grouper iridovirus, is a large double-stranded DNA virus, reaching a diameter of 220 nm and packaging a genome of 140 kb. We present a 3D cryoelectron microscopy (cryo-EM) icosahedral reconstruction of SGIV determined at 8.6-Å resolution. It reveals several layers including a T = 247 icosahedral outer coat, anchor proteins, a lipid bilayer, and the encapsidated DNA. A new segmentation tool, iSeg, was applied to extract these layers from the reconstructed map. The outer coat wa ...[more]