Dataset Information

The Prevotella copri Complex Comprises Four Distinct Clades Underrepresented in Westernized Populations.

ABSTRACT: Prevotella copri is a common human gut microbe that has been both positively and negatively associated with host health. In a cross-continent meta-analysis exploiting >6,500 metagenomes, we obtained >1,000 genomes and explored the genetic and population structure of P. copri. P. copri encompasses four distinct clades (>10% inter-clade genetic divergence) that we propose constitute the P. copri complex, and all clades were confirmed by isolate sequencing. These clades are nearly ubiquitous and co-present in non-Westernized populations. Genomic analysis showed substantial functional diversity in the complex with notable differences in carbohydrate metabolism, suggesting that multi-generational dietary modifications may be driving reduced prevalence in Westernized populations. Analysis of ancient metagenomes highlighted patterns of P. copri presence consistent with modern non-Westernized populations and a clade delineation time pre-dating human migratory waves out of Africa. These findings reveal that P. copri exhibits a high diversity that is underrepresented in Western-lifestyle populations.

SUBMITTER: Tett A

PROVIDER: S-EPMC6854460 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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The Prevotella copri Complex Comprises Four Distinct Clades Underrepresented in Westernized Populations.

Tett Adrian A Huang Kun D KD Asnicar Francesco F Fehlner-Peach Hannah H Pasolli Edoardo E Karcher Nicolai N Armanini Federica F Manghi Paolo P Bonham Kevin K Zolfo Moreno M De Filippis Francesca F Magnabosco Cara C Bonneau Richard R Lusingu John J Amuasi John J Reinhard Karl K Rattei Thomas T Boulund Fredrik F Engstrand Lars L Zink Albert A Collado Maria Carmen MC Littman Dan R DR Eibach Daniel D Ercolini Danilo D Rota-Stabelli Omar O Huttenhower Curtis C Maixner Frank F Segata Nicola N

Cell host & microbe 20191010 5

Prevotella copri is a common human gut microbe that has been both positively and negatively associated with host health. In a cross-continent meta-analysis exploiting >6,500 metagenomes, we obtained >1,000 genomes and explored the genetic and population structure of P. copri. P. copri encompasses four distinct clades (>10% inter-clade genetic divergence) that we propose constitute the P. copri complex, and all clades were confirmed by isolate sequencing. These clades are nearly ubiquitous and co ...[more]

PMID: 31607556

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Project description:BackgroundExcessive fat accumulation of pigs is undesirable, as it severely affects economic returns in the modern pig industry. Studies in humans and mice have examined the role of the gut microbiome in host energy metabolism. Commercial Duroc pigs are often fed formula diets with high energy and protein contents. Whether and how the gut microbiome under this type of diet regulates swine fat accumulation is largely unknown.ResultsIn the present study, we systematically investigated the correlation of gut microbiome with pig lean meat percentage (LMP) in 698 commercial Duroc pigs and found that Prevotella copri was significantly associated with fat accumulation of pigs. Fat pigs had significantly higher abundance of P. copri in the gut. High abundance of P. copri was correlated with increased concentrations of serum metabolites associated with obesity, e.g., lipopolysaccharides, branched chain amino acids, aromatic amino acids, and the metabolites of arachidonic acid. Host intestinal barrier permeability and chronic inflammation response were increased. A gavage experiment using germ-free mice confirmed that the P. copri isolated from experimental pigs was a causal species increasing host fat accumulation and altering serum metabolites. Colon, adipose tissue, and muscle transcriptomes in P. copri-gavaged mice indicated that P. copri colonization activated host chronic inflammatory responses through the TLR4 and mTOR signaling pathways and significantly upregulated the expression of the genes related to lipogenesis and fat accumulation, but attenuated the genes associated with lipolysis, lipid transport, and muscle growth.ConclusionsTaken together, the results proposed that P. copri in the gut microbial communities of pigs fed with commercial formula diets activates host chronic inflammatory responses by the metabolites through the TLR4 and mTOR signaling pathways, and increases host fat deposition significantly. The results provide fundamental knowledge for reducing fat accumulation in pigs through regulating the gut microbial composition. Video Abstract.

Dataset Information

The Prevotella copri Complex Comprises Four Distinct Clades Underrepresented in Westernized Populations.

Publications

The Prevotella copri Complex Comprises Four Distinct Clades Underrepresented in Westernized Populations.

Similar Datasets

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