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Melatonin Reduces Androgen Production and Upregulates Heme Oxygenase-1 Expression in Granulosa Cells from PCOS Patients with Hypoestrogenia and Hyperandrogenia.


ABSTRACT: Background/Aims:Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS is a pathophysiological syndrome caused by chronic inflammation and oxidative stress. The aim of this study was to investigate the mechanism of melatonin regulation on androgen production and antioxidative damage in granulosa cells from PCOS patients with hypoestrogenia and hyperandrogenia. Methods:Cumulus-oocyte complexes were collected from PCOS patients who had low levels of estrogen in follicular fluids. Results:Melatonin triggered upregulation of cytochrome P450 family 19 subfamily A member 1 (CYP19A1) expression via the extracellular signal-regulated kinase pathway in luteinized granulosa cells. As a result, conversion of androgen to 17?-estradiol was accelerated. We also found that melatonin significantly reduced the levels of inducible nitric oxide (NO) synthetase and NO in luteinized granulosa cells. Levels of transcripts encoding NF-E2-related factor-2 and its downstream target heme oxygenase-1 were also increased, leading to anti-inflammatory and antioxidant effects. We also found that melatonin could improve oocyte development potential. Conclusion:Our preliminary results showed that melatonin had a positive impact on oocyte quality in PCOS patients with hypoestrogenia and hyperandrogenia.

SUBMITTER: Yu K 

PROVIDER: S-EPMC6854986 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Melatonin Reduces Androgen Production and Upregulates Heme Oxygenase-1 Expression in Granulosa Cells from PCOS Patients with Hypoestrogenia and Hyperandrogenia.

Yu Kun K   Wang Rong-Xiang RX   Li Meng-Hui MH   Sun Tie-Cheng TC   Zhou Yi-Wen YW   Li Yuan-Yuan YY   Sun Li-Hua LH   Zhang Bao-Lu BL   Lian Zheng-Xing ZX   Xue Song-Guo SG   Liu Yi-Xun YX   Deng Shou-Long SL  

Oxidative medicine and cellular longevity 20191020


<h4>Background/aims</h4>Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS is a pathophysiological syndrome caused by chronic inflammation and oxidative stress. The aim of this study was to investigate the mechanism of melatonin regulation on androgen production and antioxidative damage in granulosa cells from PCOS patients with hypoestrogenia and hyperandrogenia.<h4>Methods</h4>Cumulus-oocyte comp  ...[more]

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