Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10.
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ABSTRACT:
SUBMITTER: Yokoyama T
PROVIDER: S-EPMC6856148 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10.
Yokoyama Takuya T Yukuhiro Masaki M Iwasaki Yuka Y Tanaka Chika C Sankoda Kazunari K Fujiwara Risa R Shibuta Atsushi A Higashi Taishi T Motoyama Keiichi K Arima Hidetoshi H Yoshida Kazumasa K Sugimoto Nozomi N Morimoto Hiroyuki H Kosako Hidetaka H Ohshima Takashi T Fujita Masatoshi M
Scientific reports 20191114 1
We previously reported the identification of a novel antimitotic agent with carbazole and benzohydrazide structures: N'-[(9-ethyl-9H-carbazol-3-yl)methylene]-2-iodobenzohydrazide (code number NP-10). However, the mechanism(s) underlying the cancer cell-selective inhibition of mitotic progression by NP-10 remains unclear. Here, we identified NP-10-interacting proteins by affinity purification from HeLa cell lysates using NP-10-immobilized beads followed by mass spectrometry. The results showed th ...[more]
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