Ontology highlight
ABSTRACT:
SUBMITTER: Wu X
PROVIDER: S-EPMC6856190 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Nature communications 20191114 1
Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5-20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with o ...[more]