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Propolis induces Ca²⁺ signaling in immune cells.

ABSTRACT: Propolis possesses several immunological functions. We recently generated a conditional Ca²⁺ biosensor yellow cameleon (YC3.60) transgenic mouse line and established a five-dimensional (5D) (x, y, z, time, and Ca²⁺ signaling) system for intravital imaging of lymphoid tissues, including Peyer's patches (PPs). To assess the effects of propolis on immune cells, we analyzed Ca²⁺ signaling in vitro and in vivo using CD11c-Cre/YC3.60^flox transgenic mice, in which CD11c⁺ dendritic cells (DCs) specifically express YC3.60. We found that propolis induced Ca²⁺ signaling in DCs in the PPs. Intravital imaging of PPs also showed that an intraperitoneal injection of propolis augmented Ca²⁺ signaling in CD11c⁺ cells, suggesting that propolis possesses immune-stimulating activity. Furthermore, CD11c⁺ cells in PPs in mice administrated propolis indicated an increase in Ca²⁺ signaling. Our results indicate that propolis induces immunogenicity under physiological conditions.

SUBMITTER: Adachi T

PROVIDER: S-EPMC6856514 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Propolis induces Ca2+ signaling in immune cells.

Adachi Takahiro T Yoshikawa Soichiro S Tezuka Hiroyuki H Tsuji Noriko M NM Ohteki Toshiaki T Karasuyama Hajime H Kumazawa Toshihiko T

Bioscience of microbiota, food and health 20190824 4

Propolis possesses several immunological functions. We recently generated a conditional Ca2+ biosensor yellow cameleon (YC3.60) transgenic mouse line and established a five-dimensional (5D) (x, y, z, time, and Ca2+ signaling) system for intravital imaging of lymphoid tissues, including Peyer's patches (PPs). To assess the effects of propolis on immune cells, we analyzed Ca2+ signaling in vitro and in vivo using CD11c-Cre/YC3.60flox transg ...[more]

PMID: 31763117

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Project description:Introduction: Safranal, which endows saffron its unique aroma, causes vasodilatation and has a hypotensive effect in animal studies, but the mechanisms of these effects are unknown. In this study, we investigated the mechanisms of safranal vasodilation. Methods: Isolated rat endothelium-intact or -denuded aortic rings were precontracted with phenylephrine and then relaxed with safranal. To further assess the involvement of nitric oxide, prostaglandins, guanylate cyclase, and phospholipase A2 in safranal-induced vasodilation, aortic rings were preincubated with L-NAME, indomethacin, methylene blue, or quinacrine, respectively, then precontracted with phenylephrine, and safranal concentration-response curves were established. To explore the effects of safranal on Ca2+ influx, phenylephrine and CaCl2 concentration-response curves were established in the presence of safranal. Furthermore, the effect of safranal on aortic rings in the presence of ouabain, a Na+-K+ ATPase inhibitor, was studied to explore the contribution of Na+/Ca2+ exchanger to this vasodilation. Results: Safranal caused vasodilation in endothelium-intact and endothelium-denuded aortic rings. The vasodilation was not eliminated by pretreatment with L-NAME, indomethacin, methylene blue, or quinacrine, indicating the lack of a role for NO/cGMP. Safranal significantly inhibited the maximum contractions induced by phenylephrine, or by CaCl2 in Ca2+-free depolarizing buffer. Safranal also relaxed contractions induced by ouabain, but pretreatment with safranal totally abolished the development of ouabain contractions. Discussion/Conclusion: Inhibition of Na+-K+ ATPase by ouabain leads to the accumulation of Na+ intracellularly, forcing the Na+/Ca2+ exchanger to work in reverse mode, thus causing a contraction. Inhibition of the development of this contraction by preincubation with safranal indicates that safranal inhibited the Na+/Ca2+ exchanger. We conclude that safranal vasodilation is mediated by the inhibition of calcium influx from extracellular space through L-type Ca2+ channels and by the inhibition of the Na+/Ca2+ exchanger.

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Propolis induces Ca²⁺ signaling in immune cells.

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Propolis induces Ca<sup>2+</sup> signaling in immune cells.

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