Ontology highlight
ABSTRACT:
SUBMITTER: Gu Y
PROVIDER: S-EPMC6857856 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Gu Yuan Y Zheng Fei F Wang Yuan Y Su Xiaoyun X Bai Yingguo Y Yao Bin B Huang Huoqing H Luo Huiying H
PloS one 20191115 11
Talaromyces leycettanus JCM12802 is a great producer of thermophilic glycoside hydrolases (GHs). In this study, two cellulases (TlCel5A and TlCel6A) belonging to GH5 and GH6 respectively were expressed in Pichia pastoris and functionally characterized. The enzymes had acidic and thermophilic properties, showing optimal activities at pH 3.5-4.5 and 75-80°C, and retained stable at temperatures up to 60°C and over a broad pH range of 2.0-8.0. TlCel5A and TlCel6A acted against several cellulose subs ...[more]