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Rev1 wbdR tagged vaccines against Brucella ovis.

ABSTRACT: Sheep brucellosis is a worldwide extended disease caused by B. melitensis and B. ovis, two species respectively carrying smooth or rough lipopolysaccharide. Vaccine B. melitensis Rev1 is used against B. melitensis and B. ovis but induces an anti-smooth-lipopolysaccharide response interfering with B. melitensis serodiagnosis, which precludes its use against B. ovis where B. melitensis is absent. In mice, Rev1 deleted in wbkC (Brucella lipopolysaccharide formyl-transferase) and carrying wbdR (E. coli acetyl-transferase) triggered antibodies that could be differentiated from those evoked by wild-type strains, was comparatively attenuated and protected against B. ovis, suggesting its potential as a B. ovis vaccine.

SUBMITTER: Aragon-Aranda B

PROVIDER: S-EPMC6858679 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Rev1 wbdR tagged vaccines against Brucella ovis.

Aragón-Aranda Beatriz B de Miguel María Jesús MJ Martínez-Gómez Estrella E Zúñiga-Ripa Amaia A Salvador-Bescós Miriam M Moriyón Ignacio I Iriarte Maite M Muñoz Pilar M PM Conde-Álvarez Raquel R

Veterinary research 20191115 1

Sheep brucellosis is a worldwide extended disease caused by B. melitensis and B. ovis, two species respectively carrying smooth or rough lipopolysaccharide. Vaccine B. melitensis Rev1 is used against B. melitensis and B. ovis but induces an anti-smooth-lipopolysaccharide response interfering with B. melitensis serodiagnosis, which precludes its use against B. ovis where B. melitensis is absent. In mice, Rev1 deleted in wbkC (Brucella lipopolysaccharide formyl-transferase) and carrying wbdR (E. c ...[more]

PMID: 31730501

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Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice.

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Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.

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