Ontology highlight
ABSTRACT:
SUBMITTER: Atmanli A
PROVIDER: S-EPMC6861004 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Atmanli Ayhan A Hu Dongjian D Deiman Frederik Ernst FE van de Vrugt Annebel Marjolein AM Cherbonneau François F Black Lauren Deems LD Domian Ibrahim John IJ
eLife 20191008
A fundamental goal in the biological sciences is to determine how individual cells with varied gene expression profiles and diverse functional characteristics contribute to development, physiology, and disease. Here, we report a novel strategy to assess gene expression and cell physiology in single living cells. Our approach utilizes fluorescently labeled mRNA-specific anti-sense RNA probes and dsRNA-binding protein to identify the expression of specific genes in real-time at single-cell resolut ...[more]