Dataset Information

Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types.

ABSTRACT: Floor materials in indoor environments are known to be reservoirs of microbes. We focused on examining bacterial community composition, antibiotic resistance (AR) and microbial source tracking (MST) of fecal bacteria on the floor surfaces. Swab samples were collected from carpet and vinyl floors in three different buildings (medical, veterinary, and office buildings) from high and low traffic areas. Bacterial communities were determined with 16S rRNA sequencing, and AR (tetracycline (tetQ), sulfonamide, and carbapenem (KPC)) and MST (human-, canine-, avian-, and ruminant-specific fecal bacteria) were examined with quantitative polymerase chain reaction (PCR). The results show that Proteobacteria and Actinobacteria were the most abundant phyla. Traffic level significantly affected the number of operational taxonomic units. Traffic level was a key factor for distinctive bacterial community in the medical center. Targeted ARGs were detected from all buildings and tetQ concentration was related with traffic level, and KPC was only detected from the medical center. Most of the floor surfaces showed the presence of dog-specific fecal bacteria (83%) followed by bird-specific fecal bacteria (75%). The results suggest that traffic levels affected the bacterial levels and fecal contamination is prevalent on the floor surfaces. This is the first study that reports KPC presence on the floor surfaces.

SUBMITTER: Gupta M

PROVIDER: S-EPMC6862025 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types.

Gupta Mridula M Lee Seungjun S Bisesi Michael M Lee Jiyoung J

International journal of environmental research and public health 20191028 21

Floor materials in indoor environments are known to be reservoirs of microbes. We focused on examining bacterial community composition, antibiotic resistance (AR) and microbial source tracking (MST) of fecal bacteria on the floor surfaces. Swab samples were collected from carpet and vinyl floors in three different buildings (medical, veterinary, and office buildings) from high and low traffic areas. Bacterial communities were determined with 16S rRNA sequencing, and AR (tetracycline (<i>tet</i>Q ...[more]

PMID: 31661921

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Project description:In recent years, many peatlands in Europe have been rewetted for nature conservation and global warming mitigation. However, the effects on emissions of the greenhouse gas nitrous oxide (N2O) have been found to be highly variable and driving factors are poorly understood. Therefore, we measured N2O fluxes every two weeks over three years on pairs of sites (one drained, one rewetted) of three important peatland types in North-Eastern Germany, namely, percolation fen, alder forest and coastal fen. Additionally, every three months, sources of N2O were determined using a stable isotope mapping approach. Overall, fluxes were under the very dry conditions of the study years usually small with large temporal and spatial variations. Ammonium concentrations consistently and significantly correlated positively with N2O fluxes for all sites. Cumulative fluxes were often not significantly different from zero and apart from the rewetted alder forest, which was always a source of N2O, sites showed varying cumulative emission behavior (insignificant, source, potentially sink in one case) among years. Precipitation was positively correlated with cumulative fluxes on all drained sites and the rewetted alder forest. Isotope mapping indicated that N2O was always produced by more than one process simultaneously, with the estimated contribution of denitrification varying between 20 and 80%. N2O reduction played a potentially large role, with 5 to 50% of total emissions, showing large variations among sites and over time. Overall, neither the effect of rewetting, water level nor seasonality was clearly reflected in the fluxes or sources. Emissions were concentrated in hotspots and hot moments. A better understanding of the driving factors of N2O production and reduction in (rewetted) fens is essential and stable isotope methods including measurements of 15N and 18O as well as site preferences can help foster the necessary comprehension of the underlying mechanisms. Supplementary Information The online version contains supplementary material available at 10.1007/s10705-022-10244-y.

Dataset Information

Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types.

Publications

Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types.

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