A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors.
Ontology highlight
ABSTRACT:
SUBMITTER: Dostalkova A
PROVIDER: S-EPMC6863892 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors.
Dostálková Alžběta A Hadravová Romana R Kaufman Filip F Křížová Ivana I Škach Kryštof K Flegel Martin M Hrabal Richard R Ruml Tomáš T Rumlová Michaela M
Scientific reports 20191119 1
Shortly after entering the cell, HIV-1 copies its genomic RNA into double-stranded DNA in a process known as reverse transcription. This process starts inside a core consisting of an enclosed lattice of capsid proteins that protect the viral RNA from cytosolic sensors and degradation pathways. To accomplish reverse transcription and integrate cDNA into the host cell genome, the capsid shell needs to be disassembled, or uncoated. Premature or delayed uncoating attenuates reverse transcription and ...[more]
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