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Occurrence of pepper mottle virus on tomato in India.

ABSTRACT: Tomato plants exhibiting mosaic and mottling symptoms were collected from the research field of Punjab Agricultural University during 2016-17. DAS-ELISA test using pepper mottle virus (PepMoV) antiserum showed positive reaction to 5 of 25 tomato samples collected. Further molecular characterization of virus was carried out by using potyvirus specific primer pairs corresponding to most conserved and variable genes viz., Nuclear inclusion b (NIb) and P1 respectively. RT-PCR assay confirmed the association of PepMoV with tomato crop under field conditions in India.

SUBMITTER: Sharma S

PROVIDER: S-EPMC6864010 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Occurrence of pepper mottle virus on tomato in India.

Sharma Shikha S Sharma Abhishek A Kaur Sumit Inder SI

Virusdisease 20190722 3

Tomato plants exhibiting mosaic and mottling symptoms were collected from the research field of Punjab Agricultural University during 2016-17. DAS-ELISA test using pepper mottle virus (PepMoV) antiserum showed positive reaction to 5 of 25 tomato samples collected. Further molecular characterization of virus was carried out by using potyvirus specific primer pairs corresponding to most conserved and variable genes viz., Nuclear inclusion b (NIb) and P1 respectively. RT-PCR assay confirmed the ass ...[more]

PMID: 31803817

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Project description:BackgroundTomato mottle mosaic virus (ToMMV) is a recently identified species in the genus Tobamovirus and was first reported from a greenhouse tomato sample collected in Mexico in 2013. In August 2013, ToMMV was detected on peppers (Capsicum spp.) in China. However, little is known about the molecular and biological characteristics of ToMMV.MethodsReverse transcription-polymerase chain reaction (RT-PCR) and rapid identification of cDNA ends (RACE) were carried out to obtain the complete genomic sequences of ToMMV. Sap transmission was used to test the host range and pathogenicity of ToMMV.ResultsThe full-length genomes of two ToMMV isolates infecting peppers in Yunnan Province and Tibet Autonomous Region of China were determined and analyzed. The complete genomic sequences of both ToMMV isolates consisted of 6399 nucleotides and contained four open reading frames (ORFs) encoding 126, 183, 30 and 18 kDa proteins from the 5' to 3' end, respectively. Overall similarities of the ToMMV genome sequence to those of the other tobamoviruses available in GenBank ranged from 49.6% to 84.3%. Phylogenetic analyses of the sequences of full-genome nucleotide and the amino acids of its four proteins confirmed that ToMMV was most closely related to Tomato mosaic virus (ToMV). According to the genetic structure, host of origin and phylogenetic relationships, the available 32 tobamoviruses could be divided into at least eight subgroups based on the host plant family they infect: Solanaceae-, Brassicaceae-, Cactaceae-, Apocynaceae-, Cucurbitaceae-, Malvaceae-, Leguminosae-, and Passifloraceae-infecting subgroups. The detection of ToMMV on some solanaceous, cucurbitaceous, brassicaceous and leguminous plants in Yunnan Province and other few parts of China revealed ToMMV only occurred on peppers so far. However, the host range test results showed ToMMV could infect most of the tested solanaceous and cruciferous plants, and had a high affinity for the solanaceous plants.ConclusionsThe complete nucleotide sequences of two Chinese ToMMV isolates from naturally infected peppers were verified. The tobamoviruses were divided into at least eight subgroups, with ToMMV belonging to the subgroup that infected plants in the Solanaceae. In China, ToMMV only occurred on peppers in the fields till now. ToMMV could infect the plants in family Solanaceae and Cucurbitaceae by sap transmission.

Dataset Information

Occurrence of pepper mottle virus on tomato in India.

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Occurrence of pepper mottle virus on tomato in India.

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