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Stenotrophomonas maltophilia Gd2: A potential and novel isolate for fibrinolytic enzyme production.

ABSTRACT: The bacterium with an ability to produce extracellular fibrinolytic protease was isolated and identified as Stenotrophomonas maltophilia Gd2 based on ribotyping. The in-vitro fibrinolytic profile of this enzyme depicted 73% of fibrin clot dissolution within 4?h. Fibrinolytic enzyme yield influenced by different physiological (incubation time, temperature, agitation and pH), nutritional (macronutrients such as carbon and nitrogen sources) and biological (inoculums age and inoculums concentration) parameters of fermentation which were optimized based on one-factor-at-a-time (OFAT) approach. The enzyme yield improved from 886 to 1795?FU?ml-1 upon OFAT; optimized conditions include temperature - 33?°C, pH - 8.0, incubation time - 36?h, agitation - 150 RPM, 3% v/v inoculums and age of inoculum - 18?h. Further optimization of enzyme production was achieved with implementation of Plackett-Burman media designing where the production levels increased to 3411?FU?ml-1 and noticed that peptone, pH, dextrose and K2HPO4 was found to be significant factor. This ms reports the highest fibrinolytic enzyme yield with S. maltophilia to that of literature reports.

SUBMITTER: Khursade PS

PROVIDER: S-EPMC6864133 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Stenotrophomonas maltophilia Gd2: A potential and novel isolate for fibrinolytic enzyme production.

Khursade Parag S PS Galande Sneha H SH Shiva Krishna P P Prakasham R S RS

Saudi journal of biological sciences 20181030 7

The bacterium with an ability to produce extracellular fibrinolytic protease was isolated and identified as Stenotrophomonas maltophilia Gd2 based on ribotyping. The in-vitro fibrinolytic profile of this enzyme depicted 73% of fibrin clot dissolution within 4 h. Fibrinolytic enzyme yield influenced by different physiological (incubation time, temperature, agitation and pH), nutritional (macronutrients such as carbon and nitrogen sources) and biological (inoculums age and inoculums ...[more]

PMID: 31762628

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Project description:An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experimentally for its catalytic properties and by bioinformatics analysis for its immunogenicity. The promising L-asparaginase producing-isolate was selected from 722 recovered isolates and identified as Stenotrophomonas maltophilia and deposited at Microbiological Resources Centre (Cairo Mircen) under the code EMCC2297. This isolate produces both intracellular (type I) and extracellular (type II) L-asparaginases with about 4.7 fold higher extracellular L-asparaginase productivity. Bioinformatics analysis revealed clustering of Stenotrophomonas maltophiliaL-asparaginase with those of Pseudomonas species and considerable closeness to the two commercially available L-asparaginases of E. coli and Erwinia chrysanthemi. Fourteen antigenic regions are predicted for Stenotrophomonas maltophiliaL-asparaginase versus 16 and 18 antigenic regions for the Erwinia chrysanthemi and E. coliL-asparaginases. Type II L-asparaginase productivity of the test isolate reached 4.7 IU/ml/h and exhibited maximum activity with no metal ion requirement at 37 °C, pH 8.6, 40 mM asparagine concentration and could tolerate NaCl concentration up to 500 mM and retain residual activity of 55% at 70 °C after half an hour treatment period. Application both of random mutation by gamma irradiation and Response Surface Methodology that determined 38.11 °C, 6.89 pH, 19.85 h and 179.15 rpm as optimum process parameters could improve the isolate L-asparaginase productivity. Maximum production of about 8 IU/ml/h was obtained with 0.4% dextrose, 0.1% yeast extract and 10 mM magnesium sulphate. In conclusion L-asparaginase of the recovered Stenotrophomonas maltophilia EMCC2297 isolate has characters enabling it to be used for medical therapeutic application.

Dataset Information

Stenotrophomonas maltophilia Gd2: A potential and novel isolate for fibrinolytic enzyme production.

Publications

<i>Stenotrophomonas maltophilia</i> Gd2: A potential and novel isolate for fibrinolytic enzyme production.

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