Dataset Information

Genetic variants of glutathione S-transferase and the risk of acute myeloid leukemia in a Saudi population.

ABSTRACT: Objective:This study aims to investigate the genetic association of acute myeloid leukemia and glutathione S-transferase (GST) gene polymorphisms in a Saudi population. Method:100 AML cases and 100 healthy controls were recruited from the Riyadh regional hospital. In the GST gene, GSTM1 and GSTT1 variants were genotyped by multiplex PCR, and GSTP1 variants were genotyped by PCR-RFLP analysis. Statistical analysis between AML cases and controls included anthropometric measurements and evaluation of the genotypic and allelic frequencies. Result:The null genotypes of GSTM1 and GSTT1 showed no association with AML [OR 0.56 (0.26-1.19); p?=?0.31 and OR 0.65 (0.37-1.16); p?=?0.14]. Similarly, the GSTP1 genotype and allele frequencies did not indicate any association with AML [GG?+?AG vs. AA: OR 0.75 (0.43-1.31) and p?=?0.32; GG vs. AA: OR 1.73 (0.55-5.44) and p?=?0.34; G vs. A: OR 0.95 (0.61-1.46) and p?=?0.82]. Further, a haplotype analysis between AML cases and controls did not show any positive association (p?

SUBMITTER: Farasani A

PROVIDER: S-EPMC6864205 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genetic variants of glutathione S-transferase and the risk of acute myeloid leukemia in a Saudi population.

Farasani Abdullah A

Saudi journal of biological sciences 20181221 7

<h4>Objective</h4>This study aims to investigate the genetic association of acute myeloid leukemia and glutathione S-transferase (GST) gene polymorphisms in a Saudi population.<h4>Method</h4>100 AML cases and 100 healthy controls were recruited from the Riyadh regional hospital. In the GST gene, GSTM1 and GSTT1 variants were genotyped by multiplex PCR, and GSTP1 variants were genotyped by PCR-RFLP analysis. Statistical analysis between AML cases and controls included anthropometric measurements ...[more]

PMID: 31762621

Similar Datasets

Project description:BackgroundGlutathione peroxidases (GPXs) are an enzyme family with peroxidase activity. Abnormal GPX expression is associated with carcinogenesis. However, the potential role of the GPX gene family in acute myeloid leukemia (AML) remains to be comprehensively examined.MethodsWe analyzed GPX mRNA expression levels and determined the correlation between gene expression and the prognostic value via multiple universally acknowledged databases including the Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), PROGgeneV2, UALCAN, Cancer Cell Line Encyclopedia (CCLE), and The European Bioinformatics Institute (EMBL-EBI) databases. The functional network of differentially expressed GPXs was investigated via the NetworkAnalyst platform. Correlated genes as well as kinase, microRNA (miRNA), and transcription factor (TF) targets were identified using LinkedOmics.ResultsWe observed that the transcriptional expression levels of GPX-1, -2, -4, -7, and -8 had significant difference between AML patients samples and normal samples, and that AML patients with high expression of GPX-1, -3, -4, and -7 were associated with poorer prognosis of overall survival (OS). Functional enrichment analysis showed that the differentially expressed GPXs were mainly enriched in response to oxidative stress, regulation of immune response, and inflammatory response, along with glutathione metabolism and ferroptosis. Overexpression of correlated genes, PSMB10, VPS13D, NDUFS8, ATP5D, POLR2E, and HADH were linked to adverse OS in AML. Regulatory network analysis indicated that differentially expressed GPXs regulated cell proliferation, cancer progression, apoptosis, and cell cycle signaling via pathways involving cancer-related kinases (such as DAPK1 and SRC), miRNAs (such as miR-202 and miR-181), and TFs (such as SRF and E2F1).ConclusionsOur findings offer novel insights into the differential expression and prognostic potential of the GPX family in AML, and lay a foundation for subsequent research of GPX's role in the carcinogenesis and regulatory network of AML.

Dataset Information

Genetic variants of glutathione S-transferase and the risk of acute myeloid leukemia in a Saudi population.

Publications

Genetic variants of glutathione S-transferase and the risk of acute myeloid leukemia in a Saudi population.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets