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Various Patterns of Composition and Accumulation of Steroids and Triterpenoids in Cuticular Waxes from Screened Ericaceae and Caprifoliaceae Berries during Fruit Development.

ABSTRACT: Cuticular waxes are primarily composed of two classes of lipids: compounds derived from very-long-chain fatty acids and isoprenoids, particularly triterpenoids and steroids. Isoprenoids can occur in cuticular waxes in high amounts, dominating the mixture of aliphatic long-chain hydrocarbons, while in other plants they are found in trace concentrations. Triterpenoids occurring in fruit cuticular waxes are of interest due to their potential role in the protection against biotic stresses, including pathogen infections, and their impact on the mechanical toughness of the fruit surface, maintaining fruit integrity, and post-harvest quality. The aim of the present study was the determination of the changes in the triterpenoid profile of the fruit cuticular waxes of four plant species bearing edible berries: Vaccinium myrtillus, V. vitis-idaea, and Arbutus unedo of the Ericaceae and the edible honeysuckle Lonicera caerulea of the Caprifoliaceae. Triterpenoids were identified and quantified by GC-MS/FID (gas chromatography-mass spectrometry/flame ionization detection) at three different phenological stages: young berries, berries at the onset of ripening, and mature berries. During fruit development and maturation, the triterpenoid content in cuticular waxes displayed species-specific patterns of changes. The steroid content seemed to be directly correlated with the developmental stage, with a very typical point of transition between growth and ripening being observed in all the fruit analyzed in this study.

SUBMITTER: Dashbaldan S

PROVIDER: S-EPMC6864842 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Project description:Cuticular waxes covering the plant surface play pivotal roles in helping plants adapt to changing environments. However, it is still not clear whether the responses of plant cuticular waxes to their growing environments are inheritable. We collected seeds of Medicago ruthenica (a perennial legume) populations from 30 growing sites in northern China and examined the variations of leaf cuticular waxes in a common garden experiment. Four wax genes, MrFAR3-1, MrFAR3-2, MrCER1, and MrKCS1, involved in biosynthesis of predominant wax classes (primary alcohol and alkane) and wax precursors, were isolated to test the contributions of genetic variations of the coding sequences (CDS) and the promoter sequences and epigenetic modifications. The plasticity responses of the cuticular waxes were further validated by two stress-modeling experiments (drought and enhancing ultraviolet B). Great variations in total wax coverage and abundance of wax classes or wax compounds were observed among M. ruthenica populations in a common garden experiment. Stress-modeling experiments further validated that M. ruthenica would alter leaf wax depositions under changed growing conditions. The transcriptional levels of the wax genes were positively or negatively correlated with amounts of cuticular waxes. However, the analysis of promoter methylation showed that the methylation level of the promoter region was not associated with their expressions. Although both promoter sequences and CDS showed a number of polymorphic sites, the promoters were not naturally selected and insignificant difference could be observed in the numbers and types of acting elements of the four wax genes among populations. In contrast, the CDS of the wax genes were naturally selected, with a number of missense mutations resulting in alterations of the amino acid as well as their isoelectric points and polarities, which could impact on enzyme function/activity. We conclude that long-term adaptation under certain environments would induce genetic mutation of wax biosynthesis genes, resulting in inheritable alterations of cuticular wax depositions.

Project description:Alpine meadow ecosystems are susceptible to climate changes. Still, climate impact on cuticular wax in alpine meadow plants is poorly understood. Assessing the variations of cuticular wax in alpine meadow plants across different latitudes might be useful for predicting how they may respond to climate change. We studied nine alpine meadows in a climate gradient in the east side of Qinghai-Tibetan Plateau, with mean annual temperature ranging from -7.7 to 3.2°C. In total, 42 plant species were analyzed for cuticular wax, averaged 16 plant species in each meadow. Only four plant species could be observed in all sampling meadows, including Kobresia humilis,Potentilla nivea,Anaphalis lacteal, and Leontopodium nanum. The amounts of wax compositions and total cuticular wax in the four plant species varied among sampling meadows, but no significant correlation could be observed between them and temperature, precipitation, and aridity index based on plant species level. To analyze the variations of cuticular wax on community level, we averaged the amounts of n-alkanes, aliphatic acids, primary alcohols, and total cuticular wax across all investigated plant species in each sampling site. The mean annual temperature, mean temperature in July, and aridity index were significantly correlated with the averaged amounts of wax compositions and total cuticular wax. The average chain length of n-alkanes in both plant and soil linearly increased with increased temperature, whereas reduced with increased aridity index. No significant correlation could be observed between mean annual precipitation and mean precipitation from June to August and the cuticular wax amounts and average chain length. Our results suggest that the survival of some alpine plants in specific environments might be depended on their abilities in adjusting wax deposition on plant leaves, and the alpine meadow plants as a whole respond to climate change, benefiting the stability of alpine meadow ecosystem.

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Various Patterns of Composition and Accumulation of Steroids and Triterpenoids in Cuticular Waxes from Screened Ericaceae and Caprifoliaceae Berries during Fruit Development.

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