Ontology highlight
ABSTRACT:
SUBMITTER: Povedano JM
PROVIDER: S-EPMC6866228 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Povedano Juan Manuel JM Liou Joel J Wei David D Srivatsav Ashwin A Kim Jiwoong J Xie Yang Y Nijhawan Deepak D McFadden David G DG
Cell chemical biology 20190711 9
Target identification for biologically active small molecules remains a major barrier for drug discovery. Cancer cells exhibiting defective DNA mismatch repair (dMMR) have been used as a forward genetics system to uncover compound targets. However, this approach has been limited by the dearth of cancer cell lines that harbor naturally arising dMMR. Here, we establish a platform for forward genetic screening using CRISPR/Cas9 to engineer dMMR into mammalian cells. We demonstrate the utility of th ...[more]