Dataset Information

Interleukin-34 mediated by hepatitis B virus X protein via CCAAT/enhancer-binding protein ? contributes to the proliferation and migration of hepatoma cells.

ABSTRACT:

Objectives

Interleukin-34 (IL-34) is associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). However, the role and associated mechanisms of IL-34 in HBV-related HCC remain unclear. In this study, the expression, biological function and associated mechanisms of IL-34 in HBV-related HCC cells were investigated.

Methods

IL-34 expression induced by HBV and HBV X (HBX) gene was measured in hepatoma cells. The role of CCAAT/enhancer-binding protein ? (CEBP/?) in HBX-induced IL-34 expression was examined. The signal pathways involved in the expression of CEBP/? and IL-34 induced by HBX were assessed. The role of IL-34 in the proliferation and migration of HCC cells, and related mechanisms were explored.

Results

Dependent on HBX, HBV increased IL-34 expression in hepatoma cells, and HBX upregulated and interacted with CEBP/? to enhance the activity of IL-34 promoters. CEBP/? mediated by HBX was associated with the activation of PI3-K and NF-?B pathways to promote IL-34 expression. Via CSF1-R and CD138, IL-34 promoted the proliferation and migration of hepatoma cells, and contributed to the activation of ERK and STAT3 pathways and the upregulation of Bcl-xl and c-Myc mediated by HBX.

Conclusion

We demonstrate that IL-34 contributes to HBX-mediated functional abnormality of HCC cells and provides a novel insight into the molecular mechanism of carcinogenesis mediated by HBX.

SUBMITTER: Kong F

PROVIDER: S-EPMC6869657 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Interleukin-34 mediated by hepatitis B virus X protein via CCAAT/enhancer-binding protein α contributes to the proliferation and migration of hepatoma cells.

Kong Fanyun F Zhou Kai K Zhu Ting T Lian Qi Q Tao Yukai Y Li Nan N Tu Tao T Bi Yanwei Y Yang Xiaoying X Pan Xiucheng X Li Shibao S You Hongjuan H Zheng Kuiyang K Tang Renxian R

Cell proliferation 20191016 6

<h4>Objectives</h4>Interleukin-34 (IL-34) is associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). However, the role and associated mechanisms of IL-34 in HBV-related HCC remain unclear. In this study, the expression, biological function and associated mechanisms of IL-34 in HBV-related HCC cells were investigated.<h4>Methods</h4>IL-34 expression induced by HBV and HBV X (HBX) gene was measured in hepatoma cells. The role of CCAAT/enhancer-binding protein α (CEBP/ ...[more]

PMID: 31621133

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