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Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia.

ABSTRACT: Molecular epidemiology of HIV-1 infection in treatment-naive HIV-1 infected persons from Croatia was investigated. We included 403 persons, representing 92.4% of all HIV-positive individuals entering clinical care in Croatia in 2014-2017. Overall prevalence of transmitted drug resistance (TDR) was estimated at 16.4%. Resistance to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside RTI (NNRTIs) and protease inhibitors (PIs) was found in 11.4%, 6.7% and 2.5% of persons, respectively. Triple-class resistance was determined in 2.2% of individuals. In addition, a single case (1.0%) of resistance to integrase strand-transfer inhibitors (InSTIs) was found. Deep sequencing was performed on 48 randomly selected samples and detected additional TDR mutations in 6 cases. Phylogenetic inference showed that 347/403 sequences (86.1%) were part of transmission clusters and identified forward transmission of resistance in Croatia, even that of triple-class resistance. The largest TDR cluster of 53 persons with T215S was estimated to originate in the year 1992. Our data show a continuing need for pre-treatment HIV resistance testing in Croatia. Even though a low prevalence of resistance to InSTI was observed, surveillance of TDR to InSTI should be continued.

SUBMITTER: Oroz M

PROVIDER: S-EPMC6872562 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia.

Oroz Maja M Begovac Josip J Planinić Ana A Rokić Filip F Lunar Maja M MM Zorec Tomaž Mark TM Beluzić Robert R Korać Petra P Vugrek Oliver O Poljak Mario M Lepej Snježana Židovec SŽ

Scientific reports 20191121 1

Molecular epidemiology of HIV-1 infection in treatment-naive HIV-1 infected persons from Croatia was investigated. We included 403 persons, representing 92.4% of all HIV-positive individuals entering clinical care in Croatia in 2014-2017. Overall prevalence of transmitted drug resistance (TDR) was estimated at 16.4%. Resistance to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside RTI (NNRTIs) and protease inhibitors (PIs) was found in 11.4%, 6.7% and 2.5% of persons, respective ...[more]

PMID: 31754119

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Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia.

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Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia.

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