Dataset Information

Immunoreactive insulin stability in horses at risk of insulin dysregulation.

ABSTRACT:

Background

Diseases associated with insulin dysregulation (ID), such as equine metabolic syndrome and pituitary pars intermedia dysfunction, are of interest to practitioners because of their association with laminitis. Accurate insulin concentration assessment is critical in diagnosing and managing these diseases.

Hypothesis/objectives

To determine the effect of time, temperature, and collection tube type on insulin concentrations in horses at risk of ID.

Animals

Eight adult horses with body condition score?>6/9.

Methods

In this prospective study, subjects underwent an infeed oral glucose test 2 hours before blood collection. Blood samples were divided into ethylenediaminetetraacetic acid, heparinized, or serum tubes and stored at 4 or 20°C. Tubes were centrifuged and analyzed for insulin by a chemiluminescent assay over 8?days. Changes in insulin concentrations were compared with a linear mixed effects model.

Results

An overall effect of time, tube type and temperature was identified (P = .01, P = 0.001, and P = 0.001, respectively). Serum and heparinized samples had similar concentrations for 3?days at 20°C and 8?days at 4°C; however, after 3?days at 20°C, heparinized samples had significantly higher insulin concentrations (P = .004, P = .03, and P = .03 on consecutive days). Ethylenediaminetetraacetic acid samples had significantly lower insulin concentrations regardless of time and temperature (P = .001 for all comparisons).

Conclusions and clinical importance

These results suggest an ideal protocol to determine insulin concentrations involves using serum or heparinized samples with analysis occurring within 3?days at 20°C or 8?days at 4°C.

SUBMITTER: Leschke DH

PROVIDER: S-EPMC6872612 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Publications

Immunoreactive insulin stability in horses at risk of insulin dysregulation.

Leschke Dakota H DH Muir Genevieve S GS Hodgson Jack K JK Coyle Mitchell M Horn Remona R Bertin François-René FR

Journal of veterinary internal medicine 20191016 6

<h4>Background</h4>Diseases associated with insulin dysregulation (ID), such as equine metabolic syndrome and pituitary pars intermedia dysfunction, are of interest to practitioners because of their association with laminitis. Accurate insulin concentration assessment is critical in diagnosing and managing these diseases.<h4>Hypothesis/objectives</h4>To determine the effect of time, temperature, and collection tube type on insulin concentrations in horses at risk of ID.<h4>Animals</h4>Eight adul ...[more]

PMID: 31617618

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Project description:Insulin dysregulation in horses is a metabolic condition defined by high insulin concentrations in the blood and peripheral insulin resistance. This hyperinsulinemia is often associated with severe damage in the hooves, resulting in laminitis. However, we currently lack detailed information regarding the potential involvement of particular metabolic pathways in pathophysiological causes and consequences of equine insulin dysregulation. This study aimed to assess the dynamic metabolic responses given to an oral glucose test (OGT) in insulin-sensitive and insulin-dysregulated horses by a targeted metabolomics approach to identify novel metabolites associated with insulin dysregulation.Oral glucose testing triggered alterations in serum insulin (26.28?±?4.20 vs. 422.84?±?88.86 ?IU/mL, p?<?0.001) and plasma glucose concentrations (5.00?±?0.08 vs. 9.43?±?0.44 mmol/L, p?<?0.001) comparing basal and stimulated conditions after 180 min. Metabolome analyses indicated OGT-induced changes in short-chain acylcarnitines (6.00?±?0.53 vs. 3.99?±?0.23 ?mol/L, p?<?0.001), long-chain acylcarnitines (0.13?±?0.004 vs. 0.11?±?0.002 ?mol/L, p?<?0.001) and amino acids (2.18?±?0.11 vs. 1.87?±?0.08 ?mol/L, p?<?0.05). Kynurenine concentrations increased (2.88?±?0.18 vs. 3.50?±?0.19 ?mol/L, p?<?0.01), whereas spermidine concentrations decreased during OGT (0.09?±?0.004 vs. 0.08?±?0.002 ?mol/L, p?<?0.01), indicating proinflammatory conditions after oral glucose load. Insulin dysregulation was associated with lower concentrations of trans-4-hydroxyproline (4.41?±?0.29 vs. 6.37?±?0.71 ?mol/L, p?<?0.05) and methionine sulfoxide (0.40?±?0.06 vs. 0.87?±?0.13 ?mol/L, p?<?0.01; mean?±?SEM in insulin-dysregulated vs. insulin-sensitive basal samples, respectively), two metabolites which are related to antioxidant defense mechanisms.Oral glucose application during OGT resulted in profound metabolic and proinflammatory changes in horses. Furthermore, insulin dysregulation was predicted in basal samples (without OGT) by pathways associated with trans-4-hydroxyproline and methionine sulfoxide, suggesting that oxidative stress and oxidant-antioxidant disequilibrium are contributing factors to insulin dysregulation. The present findings provide new hypotheses for future research to better understand the underlying pathophysiology of insulin dysregulation in horses.

Dataset Information

Immunoreactive insulin stability in horses at risk of insulin dysregulation.

Background

Hypothesis/objectives

Animals

Methods

Results

Conclusions and clinical importance

Publications

Immunoreactive insulin stability in horses at risk of insulin dysregulation.

Similar Datasets

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