Immunoreactive insulin stability in horses at risk of insulin dysregulation.

ABSTRACT:

Background

Diseases associated with insulin dysregulation (ID), such as equine metabolic syndrome and pituitary pars intermedia dysfunction, are of interest to practitioners because of their association with laminitis. Accurate insulin concentration assessment is critical in diagnosing and managing these diseases.

Hypothesis/objectives

To determine the effect of time, temperature, and collection tube type on insulin concentrations in horses at risk of ID.

Animals

Eight adult horses with body condition score >6/9.

Methods

In this prospective study, subjects underwent an infeed oral glucose test 2 hours before blood collection. Blood samples were divided into ethylenediaminetetraacetic acid, heparinized, or serum tubes and stored at 4 or 20°C. Tubes were centrifu

SUBMITTER: Leschke DH 

PROVIDER: S-EPMC6872612 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature