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Brain activity regulates loose coupling between mitochondrial and cytosolic Ca2+ transients.


ABSTRACT: Mitochondrial calcium ([Ca2+]mito) dynamics plays vital roles in regulating fundamental cellular and organellar functions including bioenergetics. However, neuronal [Ca2+]mito dynamics in vivo and its regulation by brain activity are largely unknown. By performing two-photon Ca2+ imaging in the primary motor (M1) and visual cortexes (V1) of awake behaving mice, we find that discrete [Ca2+]mito transients occur synchronously over somatic and dendritic mitochondrial network, and couple with cytosolic calcium ([Ca2+]cyto) transients in a probabilistic, rather than deterministic manner. The amplitude, duration, and frequency of [Ca2+]cyto transients constitute important determinants of the coupling, and the coupling fidelity is greatly increased during treadmill running (in M1 neurons) and visual stimulation (in V1 neurons). Moreover, Ca2+/calmodulin kinase II is mechanistically involved in modulating the dynamic coupling process. Thus, activity-dependent dynamic [Ca2+]mito-to-[Ca2+]cyto coupling affords an important mechanism whereby [Ca2+]mito decodes brain activity for the regulation of mitochondrial bioenergetics to meet fluctuating neuronal energy demands as well as for neuronal information processing.

SUBMITTER: Lin Y 

PROVIDER: S-EPMC6872662 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Brain activity regulates loose coupling between mitochondrial and cytosolic Ca<sup>2+</sup> transients.

Lin Yuan Y   Li Lin-Lin LL   Nie Wei W   Liu Xiaolei X   Adler Avital A   Xiao Chi C   Lu Fujian F   Wang Liping L   Han Hua H   Wang Xianhua X   Gan Wen-Biao WB   Cheng Heping H  

Nature communications 20191121 1


Mitochondrial calcium ([Ca<sup>2+</sup>]<sub>mito</sub>) dynamics plays vital roles in regulating fundamental cellular and organellar functions including bioenergetics. However, neuronal [Ca<sup>2+</sup>]<sub>mito</sub> dynamics in vivo and its regulation by brain activity are largely unknown. By performing two-photon Ca<sup>2+</sup> imaging in the primary motor (M1) and visual cortexes (V1) of awake behaving mice, we find that discrete [Ca<sup>2+</sup>]<sub>mito</sub> transients occur synchrono  ...[more]

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