Comparative Transcriptomics of Ex Vivo, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus.
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ABSTRACT:
SUBMITTER: Beckman JA
PROVIDER: S-EPMC6872769 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
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Comparative Transcriptomics of <i>Ex Vivo</i>, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus.
Beckman Joshua A JA Doherty Sean P SP Feldman Zachary B ZB Banks Emily S ES Moslehi Javid J Jaffe Iris Z IZ Hamburg Naomi M NM Sheng Quanhu Q Brown Jonathan D JD
JACC. Basic to translational science 20190923 5
In this study low-input RNA-sequencing was used to annotate the molecular identity of endothelial cells isolated and immunopurified with CD144 microbeads. Using this technique, comparative gene expression profiling from healthy subjects and patients with type 2 diabetes mellitus identified both known and novel pathways linked with EC dysfunction. Modeling of diabetes by treating cultured ECs with high glucose identified shared changes in gene expression in diabetic cells. Overall, the data demon ...[more]
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