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Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions.


ABSTRACT: Postsynaptic density protein 95 (PSD-95) is a pivotal postsynaptic scaffolding protein in excitatory neurons. Although the transport and regulation of PSD-95 in synaptic regions is well understood, dendritic transport of PSD-95 before synaptic localization still remains to be clarified. To evaluate the role of KIF5, conventional kinesin, in the dendritic transport of PSD-95 protein, we expressed a transport defective form of KIF5A (?MD) that does not contain the N-terminal motor domain. Expression of ?MD significantly decreased PSD-95 level in the dendrites. Consistently, KIF5 was associated with PSD-95 in in vitro and in vivo assays. This interaction was mediated by the C-terminal tail regions of KIF5A and the third PDZ domain of PSD-95. Additionally, the ADPDZ3 (the association domain of NMDA receptor and PDZ3 domain) expression significantly reduced the levels of PSD-95, glutamate receptor 1 (GluA1) in dendrites. The association between PSD-95 and KIF5A was dose-dependent on Staufen protein, suggesting that the Staufen plays a role as a regulatory role in the association. Taken together, our data suggest a new mechanism for dendritic transport of the AMPA receptor-PSD-95.

SUBMITTER: Yoo KS 

PROVIDER: S-EPMC6873588 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions.

Yoo Ki-Seo KS   Lee Kina K   Oh Jun-Young JY   Lee Hyoeun H   Park Hyungju H   Park Young Seok YS   Kim Hyong Kyu HK  

Molecular brain 20191121 1


Postsynaptic density protein 95 (PSD-95) is a pivotal postsynaptic scaffolding protein in excitatory neurons. Although the transport and regulation of PSD-95 in synaptic regions is well understood, dendritic transport of PSD-95 before synaptic localization still remains to be clarified. To evaluate the role of KIF5, conventional kinesin, in the dendritic transport of PSD-95 protein, we expressed a transport defective form of KIF5A (ΔMD) that does not contain the N-terminal motor domain. Expressi  ...[more]

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